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The SCN of 77 B6D2F<jats:sub>1<\/jats:sub>mice synchronized with 12 h of light and 12 h of darkness were destroyed by electrocoagulation [SCN(\u2212)], while 34 animals were sham operated. Activity and body temperature were recorded by telemetry. Blood and organs were sampled at one of six circadian times for determinations of serum corticosterone concentration, blood leukocyte count, reduced glutathione (GSH), and dihydropyrimidine dehydrogenase (DPD) mRNA expression in liver and cell cycle phase distribution of bone marrow cells. Sham-operated mice displayed significant 24-h rhythms in rest-activity and body temperature, whereas such rhythms were found in none and in 15% of the SCN(\u2212) mice, respectively. SCN lesions markedly altered the rhythmic patterns in serum corticosterone and liver GSH, which became nonsinusoidal. Liver DPD mRNA expression and bone marrow cell cycle phase distribution displayed similar 24-h sinusoidal patterns in sham-operated and SCN(\u2212) mice. These results support the existence of another light-dark entrainable pacemaker that can coordinate cellular functions in peripheral organs. They suggest that the delivery of anticancer treatments at an optimal time of day may still be beneficial, despite suppressed rest-activity or cortisol rhythms.<\/jats:p>","DOI":"10.1152\/ajpregu.00085.2004","type":"journal-article","created":{"date-parts":[[2004,6,25]],"date-time":"2004-06-25T00:25:51Z","timestamp":1088123151000},"page":"R844-R851","source":"Crossref","is-referenced-by-count":55,"title":["Persistent twenty-four hour changes in liver and bone marrow despite suprachiasmatic nuclei ablation in mice"],"prefix":"10.1152","volume":"287","author":[{"given":"Elisabeth","family":"Filipski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Verdun M.","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Christine","family":"Etienne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiaoMei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Claustrat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa G.","family":"Granda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Milano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael H.","family":"Hastings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"L\u00e9vi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00759-5"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00758-2"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5488.2344"},{"key":"R4","unstructured":"B\u00e9langer PM, Desgagne M, and Bruguerolle B.Circadian periodicity in the glutathione concentration of rat liver.Ann Rev Chronopharmacol5: 215\u2013218, 1988."},{"key":"R5","unstructured":"Bjarnason GA, Jordan RC, Wood PA, Li Q, Lincoln DW, Sothern RB, Hrushesky WJ, and Ben-David Y.Circadian expression of clock genes in human oral mucosa and skin: association with specific cell-cycle phases.Am J Pathol58: 1793\u20131801, 2001."},{"key":"R6","unstructured":"Boughattas N, L\u00e9vi F, Fournier C, Lemaigre G, Roulon A, Hecquet B, Math\u00e9 G, and Reinberg A.Circadian rhythm in toxicities and tissue uptake of 1,2-diaminocyclohexane oxalatoplatinum in mice.Cancer Res49: 3362\u20133368, 1989."},{"key":"R7","unstructured":"Boughattas NA, Levi F, Fournier C, Hecquet B, Lemaigre G, Roulon A, Mathe G, and Reinberg A.Stable circadian mechanisms of toxicity of two platinum analogs (cisplatin and carboplatin) despite repeated dosages in mice.J Pharmacol Exp Ther255: 672\u2013679, 1990."},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1081\/CBI-120002677"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)01145-4"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10765"},{"key":"R11","unstructured":"Castillo L, Milano G, Santini J, Demard F, and Pierrefite V.Analysis of retinoic acid receptor \u03b2 expression in normal and malignant laryngeal mucosa by a sensitive and routine applicable reverse transcription-polymerase chain reaction-linked immunosorbent assay method.Clin Cancer Res3: 2137\u20132142, 1997."},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.20.5.1175"},{"key":"R13","unstructured":"De Prins Jand Hequet B.Data processing in chronobiological studies. 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