{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:10:36Z","timestamp":1790727036224,"version":"4.1.0"},"reference-count":50,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science KAKENHI","doi-asserted-by":"publisher","award":["17H04753"],"award-info":[{"award-number":["17H04753"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2021,4,1]]},"abstract":"<jats:p>Transient receptor potential vanilloid 4 (TRPV4) channels exist on vascular endothelial cells and eccrine sweat gland secretory cells in human skin. Here, we assessed whether TRPV4 channels contribute to cutaneous vasodilation and sweating during whole body passive heat stress ( protocol 1) and to cutaneous vasodilation during postocclusive reactive hyperemia and local thermal hyperemia ( protocol 2). Intradermal microdialysis was employed to locally deliver pharmacological agents to forearm skin sites, where cutaneous vascular conductance (CVC) and sweat rate were assessed. In protocol 1 (12 young adults), CVC and sweat rate were increased by passive whole body heating, resulting in a body core temperature elevation of 1.2\u2009\u00b1\u20090.1\u00b0C. The elevated CVC and sweat rate assessed at sites treated with TRPV4 channel antagonist (either 200 \u00b5M HC-067047 or 125 \u00b5M GSK2193874) were not different from the vehicle control site (5% dimethyl sulfoxide). After whole body heating, the TRPV4 channel agonist (100 \u00b5M GSK1016790A) was administered to each skin site, eliciting elevations in CVC. Relative to control, this response was partly attenuated by both TRPV4 channel antagonists, confirming drug efficacy. In protocol 2 (10 young adults), CVC was increased following a 5-min arterial occlusion and during local heating from 33 to 42\u00b0C. These responses did not differ between the control and the TRPV4 channel antagonist sites (200 \u00b5M HC-067047). We show that TRPV4 channels are not required for regulating cutaneous vasodilation or sweating during a whole body passive heat stress. Furthermore, they are not required for regulating cutaneous vasodilation during postocclusive reactive hyperemia and local thermal hyperemia.<\/jats:p>","DOI":"10.1152\/ajpregu.00123.2020","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T19:50:52Z","timestamp":1603309852000},"page":"R563-R573","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":20,"title":["TRPV4 channel blockade does not modulate skin vasodilation and sweating during hyperthermia or cutaneous postocclusive reactive and thermal hyperemia"],"prefix":"10.1152","volume":"320","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9696-8291","authenticated-orcid":false,"given":"Naoto","family":"Fujii","sequence":"first","affiliation":[{"name":"Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glen 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