{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:53:44Z","timestamp":1781542424837,"version":"3.54.5"},"reference-count":0,"publisher":"American Physiological Society","funder":[{"DOI":"10.13039\/100005825","name":"USDA | National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2020-02716"],"award-info":[{"award-number":["2020-02716"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"USDA | National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2019-07859"],"award-info":[{"award-number":["2019-07859"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"abstract":"<jats:p>Environment-induced heat stress (HS) is a significant and expanding threat to human health and efficient agricultural production. In this investigation, we aimed to determine the extent to which HS impacted skeletal muscle health. We hypothesized that HS would decrease autophagosome degradation and autophagic flux in mouse skeletal muscle. To test this hypothesis, female C57 mice were exposed to thermoneutral (TN; 30 \u00b0C) or heat stress (HS; 37.5 \u00b0C) conditions for 24 h, and the gastrocnemius, soleus, and diaphragm muscles were immediately collected. A subset of mice was treated with colchicine to assess autophagic flux. Environment-induced HS increased rectal and subcutaneous temperatures by ~2.0 \u00b0C and decreased feed intake by 75% and body mass by 14%, without a change in muscle mass. In all muscles, 24 h of HS increased phosphorylated HSP 27 by 1.47-fold to 4.5-fold compared to TN; however, HSPs 27, 60, 72, and 90 were similar between groups. We discovered HS increased autophagic flux in the gastrocnemius and diaphragm, but not soleus, without changes in most key autophagy regulatory proteins. Markers of mitophagy, regulators of mitochondrial architecture, and mitochondrial abundance were largely similar between groups for all muscles. Environment-induced HS increased ER stress regulators, BIP by 1.7-fold to 2.9-fold and p-IRE1\u03b1 by 1.84-fold to 2.75-fold in all muscles, and p-eIF2\u03b1 was increased 3.6-fold in soleus. Counter to our hypothesis, these data demonstrate that 24 h of HS did not decrease autophagosome degradation or flux across a range of muscles in a mouse HS model.<\/jats:p>","DOI":"10.1152\/ajpregu.00190.2025","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:09:58Z","timestamp":1781539798000},"update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Prolonged heat stress induces autophagy in mouse skeletal muscle"],"prefix":"10.1152","author":[{"given":"Sau Qwan","family":"Yap","sequence":"first","affiliation":[{"name":"Iowa State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8814-9130","authenticated-orcid":false,"given":"Melissa","family":"Roths","sequence":"additional","affiliation":[{"name":"Iowa State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6595-0862","authenticated-orcid":false,"given":"Swathy","family":"Krishna","sequence":"additional","affiliation":[{"name":"Iowa State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0071-3684","authenticated-orcid":false,"given":"Tori E.","family":"Rudolph","sequence":"additional","affiliation":[{"name":"Iowa State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3797-7539","authenticated-orcid":false,"given":"Joshua T.","family":"Selsby","sequence":"additional","affiliation":[{"name":"Iowa State University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","published-online":{"date-parts":[[2026,6,15]]},"container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpregu.00190.2025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:09:58Z","timestamp":1781539798000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpregu.00190.2025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,15]]},"references-count":0,"alternative-id":["10.1152\/ajpregu.00190.2025"],"URL":"https:\/\/doi.org\/10.1152\/ajpregu.00190.2025","relation":{},"ISSN":["0363-6119","1522-1490"],"issn-type":[{"value":"0363-6119","type":"print"},{"value":"1522-1490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,15]]},"assertion":[{"value":"2025-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ajpregu.00190.2025"}}