{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:33:37Z","timestamp":1758710017834},"reference-count":48,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2014,9,15]]},"abstract":"<jats:p> Somatostain (SS) is known to inhibit growth hormone (GH) and prolactin (PRL) secretion. Somatolactin (SL) is a member of the GH\/PRL family, but its regulation by goldfish brain somatostatin-28 (gbSS-28) has not been examined. To this end, the structural identity of goldfish SL\u03b1 was established by 5\u2032\/3\u2032-rapid amplification of cDNA ends. As revealed by in situ hybridization and immunohistochemical staining, the expression of SL isoforms was detected in pituitary cells located in the neurointermediate lobe (NIL). The transcripts of goldfish SS receptor 5a (Sst5a) but not Sst1b, Sst2, or Sst3a were detected in the goldfish NIL cells by RT-PCR. In goldfish pituitary cells, gbSS-28 not only had an inhibitory effect on basal SL\u03b1 and SL\u03b2 mRNA levels but also could abolish insulin-like growth factor-stimulated SL gene expression. In primary cultures of goldfish NIL cells, gbSS-28 reduced forskolin-stimulated total cAMP production. With the use of a pharmacological approach, the adenylate cyclase (AC)\/cAMP and phospholipase C (PLC)\/inositol trisphosphate (IP<jats:sub>3<\/jats:sub>)\/protein kinase C (PKC) cascades were shown to be involved in gbSS-28-inhibited SL\u03b1 mRNA expression. Similar postreceptor signaling cascades were also observed for gbSS-28-reduced SL\u03b2 mRNA expression, except that PKC coupling to PLC was not involved. These results provide evidence that gbSS-28 can inhibit SL\u03b1 and SL\u03b2 gene expression at the goldfish pituitary level via Sst5 through differential coupling of AC\/cAMP and PLC\/IP<jats:sub>3<\/jats:sub>\/PKC cascades. <\/jats:p>","DOI":"10.1152\/ajpregu.00193.2014","type":"journal-article","created":{"date-parts":[[2014,7,10]],"date-time":"2014-07-10T03:58:50Z","timestamp":1404964730000},"page":"R755-R768","source":"Crossref","is-referenced-by-count":4,"title":["Somatostatin-28 inhibitory action on somatolactin-\u03b1 and -\u03b2 gene expression in goldfish"],"prefix":"10.1152","volume":"307","author":[{"given":"Quan","family":"Jiang","sequence":"first","affiliation":[{"name":"Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Peoples Republic of China; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson O. L.","family":"Wong","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Hong Kong, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-1857"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1996.0178"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19990101)44:1<19::AID-JEMT4>3.0.CO;2-L"},{"key":"B4","first-page":"540","volume":"75","author":"Bertherat J","year":"1992","journal-title":"J Clin Endocrinol Metab"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1560441"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0622-8"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2007.06.010"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1139\/o00-011"},{"key":"B9","doi-asserted-by":"crossref","unstructured":"Chen tt MA, Shamblott M, Chan KM, Tang YL, Cheng CM, Yang BY. Structure and Evolution of Fish Growth Hormone and Insulin-Like Growth Factor Genes. New York, NY: Academic, 1994, p. 179\u2013209.","DOI":"10.1016\/S1546-5098(08)60067-9"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6271"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00035.2006"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.048819"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05511.x"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1210\/endo-110-3-910"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0378"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90386.2008"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90385.2008"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00347.2011"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1996.6821"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1996.6799"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2005.10.011"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00186.2005"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2265.2002.01662.x"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.11.7094"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(00)00278-1"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00745-6"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(02)00276-0"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1995.268.3.R577"},{"key":"B29","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0305-0491(97)00357-X","volume":"119","author":"Melamed P","year":"1998","journal-title":"Comp Biochem Physiol"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.2.511"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2004.12.002"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(01)01626-0"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.11.4330"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2005.03.003"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-6480(03)00097-2"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(92)90142-7"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1021\/bi00220a010"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-3-1380"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2013.07.005"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.41.437"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.10.025"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1007\/BF00296636"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2009.06.001"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.11.007"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2004.01218.x"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0439"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1820509"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1996.6810"}],"container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpregu.00193.2014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T17:34:54Z","timestamp":1567964094000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpregu.00193.2014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,15]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,9,15]]}},"alternative-id":["10.1152\/ajpregu.00193.2014"],"URL":"https:\/\/doi.org\/10.1152\/ajpregu.00193.2014","relation":{},"ISSN":["0363-6119","1522-1490"],"issn-type":[{"value":"0363-6119","type":"print"},{"value":"1522-1490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,15]]}}}