{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:58:31Z","timestamp":1787162311535,"version":"build-2736575974"},"reference-count":88,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>\n                    The K,Cl cotransporters (KCCs) of the SLC12 superfamily play critical roles in the regulation of cell volume, concentrations of intracellular Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    , and epithelial transport in vertebrate tissues. To date, the role(s) of KCCs in the renal functions of mosquitoes and other insects is less clear. In the present study, we sought molecular and functional evidence for the presence of a KCC in renal (Malpighian) tubules of the mosquito Aedes aegypti . Using RT-PCR on Aedes Malpighian tubules, we identified five alternatively spliced partial cDNAs that encode putative SLC12-like KCCs. The majority transcript is AeKCC1-A\n                    <jats:sub>1<\/jats:sub>\n                    ; its full-length cDNA was cloned. After expression of the AeKCC1-A protein in Xenopus oocytes, the Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    -dependent uptake of\n                    <jats:sup>86<\/jats:sup>\n                    Rb\n                    <jats:sup>+<\/jats:sup>\n                    is 1) activated by 1 mM N-ethylmaleimide and cell swelling, 2) blocked by 100 \u03bcM dihydroindenyloxyalkanoic acid (DIOA), and 3) dependent upon N-glycosylation of AeKCC1-A. In Aedes Malpighian tubules, AeKCC1 immunoreactivity localizes to the apical brush border of principal cells, which are the predominant cell type in the epithelium. In vitro physiological assays of Malpighian tubules show that peritubular DIOA (10 \u03bcM): 1) significantly reduces both the control and diuretic rates of transepithelial fluid secretion and 2) has negligible effects on the membrane voltage and input resistance of principal cells. Taken together, the above observations indicate the presence of a KCC in the apical membrane of principal cells where it participates in a major electroneutral transport pathway for the transepithelial secretion of fluid in this highly electrogenic epithelium.\n                  <\/jats:p>","DOI":"10.1152\/ajpregu.00223.2011","type":"journal-article","created":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T02:06:03Z","timestamp":1312423563000},"page":"R1318-R1337","source":"Crossref","is-referenced-by-count":33,"title":["Role of an apical K,Cl cotransporter in urine formation by renal tubules of the yellow fever mosquito (Aedes aegypti)"],"prefix":"10.1152","volume":"301","author":[{"given":"Peter M.","family":"Piermarini","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca 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