{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T22:59:19Z","timestamp":1771541959730,"version":"3.50.1"},"reference-count":102,"publisher":"American Physiological Society","issue":"2","funder":[{"name":"Science without Borders","award":["8938-13-0"],"award-info":[{"award-number":["8938-13-0"]}]},{"name":"Science without Borders","award":["401061\/2014-0"],"award-info":[{"award-number":["401061\/2014-0"]}]},{"DOI":"10.13039\/501100001807","name":"S&#x00E3;o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2012\/16537-0"],"award-info":[{"award-number":["2012\/16537-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"MCTI | Conselho Nacional de Desenvolvimento Cient&#x00ED;fico e Tecnol&#x00F3;gico","doi-asserted-by":"publisher","award":["168061\/2017-1"],"award-info":[{"award-number":["168061\/2017-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2020,8,1]]},"abstract":"<jats:p>Vascular tone in the reptilian pulmonary vasculature is primarily under cholinergic, muscarinic control exerted via the vagus nerve. This control has been ascribed to a sphincter located at the arterial outflow, but we speculated whether the vascular control in the pulmonary artery is more widespread, such that responses to acetylcholine and electrical stimulation, as well as the expression of muscarinic receptors, are prevalent along its length. Working on the South American rattlesnake ( Crotalus durissus), we studied four different portions of the pulmonary artery (truncus, proximal, distal, and branches). Acetylcholine elicited robust vasoconstriction in the proximal, distal, and branch portions, but the truncus vasodilated. Electrical field stimulation (EFS) caused contractions in all segments, an effect partially blocked by atropine. We identified all five subtypes of muscarinic receptors (M<jats:sub>1<\/jats:sub>\u2013M<jats:sub>5<\/jats:sub>). The expression of the M<jats:sub>1<\/jats:sub>receptor was largest in the distal end and branches of the pulmonary artery, whereas expression of the muscarinic M<jats:sub>3<\/jats:sub>receptor was markedly larger in the truncus of the pulmonary artery. Application of the neural tracer 1,1\u2032-dioctadecyl-3,3,3\u2032,3\u2032-tetramethylindo-carbocyanine perchlorate (DiI) revealed widespread innervation along the whole pulmonary artery, and retrograde transport of the same tracer indicated two separate locations in the brainstem providing vagal innervation of the pulmonary artery, the medial dorsal motor nucleus of the vagus and a ventro-lateral location, possibly constituting a nucleus ambiguus. These results revealed parasympathetic innervation of a large portion of the pulmonary artery, which is responsible for regulation of vascular conductance in C. durissus, and implied its integration with cardiorespiratory control.<\/jats:p>","DOI":"10.1152\/ajpregu.00310.2019","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T12:46:29Z","timestamp":1592397989000},"page":"R156-R170","source":"Crossref","is-referenced-by-count":12,"title":["Cholinergic regulation along the pulmonary arterial tree of the South American rattlesnake: vascular reactivity, muscarinic receptors, and vagal innervation"],"prefix":"10.1152","volume":"319","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5436-7102","authenticated-orcid":false,"given":"Renato","family":"Filogonio","sequence":"first","affiliation":[{"name":"Zoophysiology, Department of Bioscience, Aarhus University, Aarhus, Denmark"},{"name":"Department of Physiological Sciences, Federal University of S\u00e3o Carlos, S\u00e3o Carlos, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina R.","family":"Sartori","sequence":"additional","affiliation":[{"name":"Department of Zoology, State University of S\u00e3o Paulo, Rio Claro, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susie","family":"Morgensen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Pulmonary, and Cardiovascular Pharmacology, Aarhus University, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Driele","family":"Tavares","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Federal University of S\u00e3o Carlos, S\u00e3o Carlos, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Campos","sequence":"additional","affiliation":[{"name":"Superior Institute of Biomedical Sciences, Cear\u00e1 State University, Fortaleza, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Augusto S.","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Zoology, State University of S\u00e3o Paulo, Rio Claro, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin W.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Federal University of S\u00e3o Carlos, S\u00e3o Carlos, Brazil"},{"name":"School of Biosciences, University of Birmingham, Birmingham, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerson J.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Federal University of S\u00e3o Carlos, S\u00e3o Carlos, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto","family":"De Nucci","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Department of Pharmacology, University of Campinas, Campinas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf","family":"Simonsen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Pulmonary, and Cardiovascular Pharmacology, Aarhus University, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e9o A. C.","family":"Leite","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Federal University of S\u00e3o Carlos, S\u00e3o Carlos, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Wang","sequence":"additional","affiliation":[{"name":"Zoophysiology, Department of Bioscience, Aarhus University, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2005.10.001"},{"key":"B2","first-page":"87","volume":"47","author":"Barnes PJ","year":"1995","journal-title":"Pharmacol Rev"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/0010-4035(72)90048-1"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0010-4035(73)90014-1"},{"key":"B5","first-page":"1","volume-title":"Biology of the Reptilia, Volume 10, Nurology B","author":"Berger PJ","year":"1979"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1126\/science.3037705"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90190-0"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117257"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12572"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689039"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/27.1.5"},{"key":"B12","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1242\/jeb.96.1.343","volume":"96","author":"Burggren WW","year":"1982","journal-title":"J Exp Biol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/0010-4035(71)90053-X"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02278"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2019.04.015"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0203573"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196123"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183766"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2011.02246.x"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1994.267.2.R569"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199904260-00004"},{"key":"B22","doi-asserted-by":"crossref","first-page":"3367","DOI":"10.1242\/jeb.201.24.3367","volume":"201","author":"Crossley D","year":"1998","journal-title":"J Exp Biol"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(20000601)286:7<683:AID-JEZ2>3.0.CO;2-4"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051980104"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1007\/BF00224724"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1007\/BF00218794"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1007\/BF00691713"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019583"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2016.12.030"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2017.02.005"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2017.05.005"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.2994\/SAJH-D-18-00013.1"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.177766"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-016-1008-y"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1985.59.2.285"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1007\/BF00335293"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-005-0476-2"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.06.420"},{"key":"B39","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.1242\/jeb.202.24.3647","volume":"202","author":"Gamperl AK","year":"1999","journal-title":"J Exp Biol"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.179820"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074310"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/nature03444"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.268.5.H1862"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1086\/physzool.67.6.30163900"},{"key":"B45","first-page":"425","volume-title":"Biology of the Reptilia, Morphology G\u2013Visceral Organs","volume":"19","author":"Hicks JW","year":"1998"},{"key":"B46","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1242\/jeb.199.6.1435","volume":"199","author":"Hicks JW","year":"1996","journal-title":"J Exp Biol"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19960601\/15)275:2\/3<204:AID-JEZ12>3.0.CO;2-J"},{"key":"B48","first-page":"207","volume-title":"Ontogeny and Phylogeny of the Vertebrate Heart","author":"Hicks JW"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2015.04.014"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.1.171"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(83)91312-4"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.30.040190.003221"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12056"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.7.6.828"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.1968.tb10939.x"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2014.04.002"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.215335"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb14903.x"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000130661.82773.ca"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.109025"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00880-2"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.2.C519"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1126\/science.2749262"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1086\/physzool.67.6.30163897"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1921.56.1.72"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1961.201.6.1109"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1016\/0010-406X(67)90773-6"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1139\/z77-049"},{"key":"B69","first-page":"83","volume-title":"Physiology of Elasmobranch Fishes: B Internal Processes","author":"Milsom WK","year":"2016"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2011.11.003"},{"key":"B71","first-page":"193","volume-title":"Comparative Physiology and the Evolution of the Autonomic Nervous System","author":"Morris JL","year":"1994"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.109.130880"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.41.1.19"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1827.1999.00977.x"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1213\/01.ANE.0000082243.98617.3B"},{"key":"B76","first-page":"71","volume":"3","author":"Rao X","year":"2013","journal-title":"Biostat Bioinforma Biomath"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.023440"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199312000-00010"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.197954"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"B81","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1242\/jeb.64.2.323","volume":"64","author":"Shelton G","year":"1976","journal-title":"J Exp Biol"},{"key":"B82","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(01)00726-9"},{"key":"B83","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13720.x"},{"key":"B84","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050896"},{"key":"B85","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00200.2005"},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2005.05.049"},{"key":"B87","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2004.12.016"},{"key":"B88","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051920308"},{"key":"B89","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.024042"},{"key":"B90","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.3.855"},{"key":"B91","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.086199"},{"key":"B92","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2010007500044"},{"key":"B93","first-page":"3","volume-title":"Pulmonary Vascular Physiology and Pathophysiology","author":"Tenney SM","year":"1989"},{"key":"B94","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20429"},{"key":"B95","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(88)90081-7"},{"key":"B96","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00681"},{"key":"B97","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1242\/jeb.199.8.1791","volume":"199","author":"Wang T","year":"1996","journal-title":"J Exp Biol"},{"key":"B98","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-445X.2001.tb00044.x"},{"key":"B99","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199909)22:9<1177:AID-MUS3>3.0.CO;2-P"},{"key":"B100","doi-asserted-by":"publisher","DOI":"10.1086\/physzool.65.2.30158254"},{"key":"B101","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251542998"},{"key":"B102","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.115.223693"}],"container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpregu.00310.2019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T01:15:02Z","timestamp":1667006102000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpregu.00310.2019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,1]]},"references-count":102,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,8,1]]}},"alternative-id":["10.1152\/ajpregu.00310.2019"],"URL":"https:\/\/doi.org\/10.1152\/ajpregu.00310.2019","relation":{},"ISSN":["0363-6119","1522-1490"],"issn-type":[{"value":"0363-6119","type":"print"},{"value":"1522-1490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,1]]}}}