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Transmission electron microscopy analysis of both the PNA<jats:sup>+<\/jats:sup>and PNA negative (PNA<jats:sup>\u2212<\/jats:sup>) fraction showed that PNA binds to MR chloride cells while the PNA<jats:sup>\u2212<\/jats:sup>cell fraction is comprised of MR cells with features characteristic of pavement cells. Western blotting demonstrated that both PNA<jats:sup>+<\/jats:sup>and PNA<jats:sup>\u2212<\/jats:sup>fractions had high levels of Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase and Sco1 expression; however, relative expression of H<jats:sup>+<\/jats:sup>-ATPase in PNA<jats:sup>+<\/jats:sup>and PNA<jats:sup>\u2212<\/jats:sup>cells demonstrated that untreated fish had twofold higher H<jats:sup>+<\/jats:sup>-ATPase levels in PNA<jats:sup>\u2212<\/jats:sup>cells relative to the PNA<jats:sup>+<\/jats:sup>cells. 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