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However, the precise role of IFN\u03b3 in contributing to PE pathophysiology remains unclear. Using the reduced uterine perfusion pressure (RUPP) rat model of placental ischemia, we tested the hypothesis that neutralization of IFN\u03b3 in RUPPs will decrease placental reactive oxygen species (ROS) and improve vascular function resulting in decreased MAP and improved fetal growth. On gestation day ( GD) 14, the RUPP procedure was performed and on GDs 15 and 18, a subset of normal pregnant rats (NP) and RUPP rats were injected with 10\u2009\u03bcg\/kg of an anti-rat IFN\u03b3 monoclonal antibody. On GD 18, uterine artery resistance index (UARI) was measured via Doppler ultrasound and on GD 19, mean arterial pressure (MAP) was measured, animals were euthanized, and blood and tissues were collected for analysis. Increased MAP was observed in RUPP rats compared with NP and was reduced in RUPP + anti-IFN\u03b3. Placental ROS was also increased in RUPP rats compared with NP rats and was normalized in RUPP + anti-IFN\u03b3. Fetal and placental weights were reduced in RUPP rats, but were not improved following anti-IFN\u03b3 treatment. However, UARI was elevated in RUPP compared with NP rats and was reduced in RUPP + anti-IFN\u03b3. In conclusion, we observed that IFN\u03b3 neutralization reduced MAP, UARI, and placental ROS in RUPP recipients. These data suggest that IFN\u03b3 is a potential mechanism by which cNKs contribute to PE pathophysiology and may represent a therapeutic target to improve maternal outcomes in PE. <\/jats:p>","DOI":"10.1152\/ajpregu.00349.2020","type":"journal-article","created":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T12:00:28Z","timestamp":1622635228000},"page":"R112-R124","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Interferon \u03b3 neutralization reduces blood pressure, uterine artery resistance index, and placental oxidative stress in placental ischemic rats"],"prefix":"10.1152","volume":"321","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7392-9054","authenticated-orcid":true,"given":"Olivia K.","family":"Travis","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, 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