{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:38:39Z","timestamp":1765294719028,"version":"3.41.0"},"reference-count":34,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>The localization of dopamine stores and the expression and localization of dopamine (DAT) and vesicular monoamine transporters (VMAT) type-1 and -2 and of dopamine D<jats:sub>1<\/jats:sub>-like and D<jats:sub>2<\/jats:sub>-like receptor subtypes were investigated in rat submandibular, sublingual, and parotid salivary glands by HPLC with electrochemical detection, as well as immunochemical and immunohistochemical techniques. Male Wistar rats of 2 mo of age were used. The highest dopamine levels were measured in the parotid gland, followed by the submandibular and sublingual glands. Western blot analysis revealed DAT, VMAT-1, VMAT-2, and dopamine receptors immunoreactivity in membrane preparations obtained from the three glands investigated. Immunostaining for dopamine and transporters was developed within striated ducts. Salivary glands processed for dopamine receptors immunohistochemistry developed an immunoreaction primarily in striated and excretory ducts. In the submandibular gland, acinar cells displayed strong immunoreactivity for the D<jats:sub>2<\/jats:sub>receptor, while cells of the convoluted granular tubules were negative for both D<jats:sub>1<\/jats:sub>-like and D<jats:sub>2<\/jats:sub>-like receptors. Parotid glands acinar cells displayed the highest immunoreactivity for both D<jats:sub>1<\/jats:sub>and D<jats:sub>2<\/jats:sub>receptors compared with other salivary glands. The above localization of dopamine and dopaminergic markers investigated did not correspond closely with neuron-specific enolase (NSE) localization. This indicates that at least in part, catecholamine stores and dopaminergic markers are independent from glandular innervation. These findings suggest that rat major salivary glands express a dopaminergic system probably involved in salivary secretion. The stronger immunoreactivity for dopamine transporters and receptors in striated duct cells suggests that the dopaminergic system could regulate not only quality, but also volume and ionic concentration of saliva.<\/jats:p>","DOI":"10.1152\/ajpregu.00455.2014","type":"journal-article","created":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T04:11:00Z","timestamp":1435810260000},"page":"R585-R593","source":"Crossref","is-referenced-by-count":10,"title":["Dopamine, vesicular transporters, and dopamine receptor expression in rat major salivary glands"],"prefix":"10.1152","volume":"309","author":[{"given":"Daniele","family":"Tomassoni","sequence":"first","affiliation":[{"name":"Scuola di Bioscienze e Medicina Veterinaria, Universit\u00e0 di Camerino, Camerino Italy;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enea","family":"Traini","sequence":"additional","affiliation":[{"name":"Scuola di Scienze del Farmaco e dei Prodotti della Salute, Universit\u00e0 di Camerino, Camerino Italy; 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