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We hypothesized that hypoxia exerts a direct effect on the mesenteric artery (MA) that may contribute to this response. Using wire myography, we investigated the response to hypoxia (Po<jats:sub>2<\/jats:sub> ~2.5 kPa for 20 min) of isolated MAs from 15- to 21-day chicken embryos (E15, E19, E21), and 1- to 45-day-old chickens (P1, P3, P14, P45). Agonist-induced pretone or an intact endothelium were not required to obtain a consistent and reproducible response to hypoxia, which showed a pattern of initial rapid phasic contraction followed by a sustained tonic contraction. Phasic contraction was reduced by elimination of extracellular Ca<jats:sup>2+<\/jats:sup> or by presence of the neurotoxin tetrodotoxin, the \u03b1<jats:sub>1<\/jats:sub>-adrenoceptor antagonist prazosin, or inhibitors of L-type voltage-gated Ca<jats:sup>2+<\/jats:sup> channels (nifedipine), mitochondrial electron transport chain (rotenone and antimycin A), and NADPH oxidase (VAS2870). The Rho-kinase inhibitor Y27632 impaired both phasic and tonic contraction and, when combined with elimination of extracellular Ca<jats:sup>2+<\/jats:sup>, hypoxia-induced contraction was virtually abolished. Hypoxic MA contraction was absent at E15 but present from E19 and increased toward the first days posthatching. It then decreased during the first weeks of life and P45 MAs were unable to sustain hypoxia-induced contraction over time. In conclusion, the results of the present study demonstrate that hypoxic vasoconstriction is an intrinsic feature of chicken MA vascular smooth muscle cells during late embryogenesis and the perinatal period. <\/jats:p>","DOI":"10.1152\/ajpregu.00461.2015","type":"journal-article","created":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T03:03:52Z","timestamp":1471489432000},"page":"R858-R869","source":"Crossref","is-referenced-by-count":11,"title":["Hypoxia-induced contraction of chicken embryo mesenteric arteries: mechanisms and developmental changes"],"prefix":"10.1152","volume":"311","author":[{"given":"Leonie","family":"Brinks","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Maastricht University Medical Center (MUMC+), School for Oncology and Developmental Biology (GROW), Maastricht, The Netherlands;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rob M. 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