{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T15:48:15Z","timestamp":1750088895502,"version":"3.37.3"},"reference-count":20,"publisher":"American Physiological Society","issue":"8","funder":[{"DOI":"10.13039\/100007217","name":"Health Services Research and Development (Veterans Health Administration HSR and D)","doi-asserted-by":"publisher","award":["CDA2 1BX001299"],"award-info":[{"award-number":["CDA2 1BX001299"]}],"id":[{"id":"10.13039\/100007217","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI)","doi-asserted-by":"publisher","award":["K01 HL-125503"],"award-info":[{"award-number":["K01 HL-125503"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)","doi-asserted-by":"publisher","award":["R21 DK-105368"],"award-info":[{"award-number":["R21 DK-105368"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2016,4,15]]},"abstract":"<jats:p> Adipose tissue (AT) inflammation is a hallmark characteristic of obesity and an important determinant of insulin resistance and cardiovascular disease; therefore, a better understanding of factors regulating AT inflammation is critical. It is well established that reduced vascular endothelial nitric oxide (NO) bioavailability promotes arterial inflammation; however, the role of NO in modulating inflammation in AT remains disputed. In the present study, 10-wk-old C57BL6 wild-type and endothelial nitric oxide synthase (eNOS) knockout male mice were randomized to either a control diet (10% kcal from fat) or a Western diet (44.9% kcal from fat, 17% sucrose, and 1% cholesterol) for 18 wk ( n = 7 or 8\/group). In wild-type mice, Western diet-induced obesity led to increased visceral white AT expression of inflammatory genes (e.g., MCP1, TNF-\u03b1, and CCL5 mRNAs) and markers of macrophage infiltration (e.g., CD68, ITGAM, EMR1, CD11C mRNAs, and Mac-2 protein), as well as reduced markers of mitochondrial content (e.g., OXPHOS complex I and IV protein). Unexpectedly, these effects of Western diet on visceral white AT were not accompanied by decreases in eNOS phosphorylation at Ser-1177 or increases in eNOS phosphorylation at Thr-495. Also counter to expectations, eNOS knockout mice, independent of the diet, were leaner and did not exhibit greater white or brown AT inflammation compared with wild-type mice. Collectively, these findings do not support the hypothesis that reduced NO production from eNOS contributes to obesity-related AT inflammation. <\/jats:p>","DOI":"10.1152\/ajpregu.00473.2015","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T00:39:11Z","timestamp":1455151151000},"page":"R744-R751","source":"Crossref","is-referenced-by-count":8,"title":["Ablation of eNOS does not promote adipose tissue inflammation"],"prefix":"10.1152","volume":"310","author":[{"given":"Thomas J.","family":"Jurrissen","sequence":"first","affiliation":[{"name":"Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan D.","family":"Sheldon","sequence":"additional","affiliation":[{"name":"Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri;"},{"name":"Research Service-Harry S. Truman Memorial Veterans Affairs Medical Center, Columbia, Missouri;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle L.","family":"Gastecki","sequence":"additional","affiliation":[{"name":"Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makenzie L.","family":"Woodford","sequence":"additional","affiliation":[{"name":"Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terese M.","family":"Zidon","sequence":"additional","affiliation":[{"name":"Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Scott","family":"Rector","sequence":"additional","affiliation":[{"name":"Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri;"},{"name":"Research Service-Harry S. 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