{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:51:59Z","timestamp":1774464719450,"version":"3.50.1"},"reference-count":63,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:p>Vascular hypoxia sensing is transduced into vasoconstriction in the pulmonary circulation, whereas systemic arteries dilate. Mitochondrial electron transport chain (mETC), reactive O<jats:sub>2<\/jats:sub>species (ROS), and K<jats:sup>+<\/jats:sup>channels have been implicated in the sensing\/signaling mechanisms of hypoxic relaxation in mammalian systemic arteries. We aimed to investigate their putative roles in hypoxia-induced relaxation in fetal chicken (19 days of incubation) femoral arteries mounted in a wire myograph. Acute hypoxia (Po<jats:sub>2<\/jats:sub>\u223c2.5 kPa) relaxed the contraction induced by norepinephrine (1 \u03bcM). Hypoxia-induced relaxation was abolished or significantly reduced by the mETC inhibitors rotenone (complex I), myxothiazol and antimycin A (complex III), and NaN<jats:sub>3<\/jats:sub>(complex IV). The complex II inhibitor 3-nitroproprionic acid enhanced the hypoxic relaxation. In contrast, the relaxations mediated by acetylcholine, sodium nitroprusside, or forskolin were not affected by the mETC blockers. Hypoxia induced a slight increase in ROS production (as measured by 2,7-dichlorofluorescein-fluorescence), but hypoxia-induced relaxation was not affected by scavenging of superoxide (polyethylene glycol-superoxide dismutase) or H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(polyethylene glycol-catalase) or by NADPH-oxidase inhibition (apocynin). Also, the K<jats:sup>+<\/jats:sup>channel inhibitors tetraethylammonium (nonselective), diphenyl phosphine oxide-1 (voltage-gated K<jats:sup>+<\/jats:sup>channel 1.5), glibenclamide (ATP-sensitive K<jats:sup>+<\/jats:sup>channel), iberiotoxin (large-conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup>channel), and BaCl<jats:sub>2<\/jats:sub>(inward-rectifying K<jats:sup>+<\/jats:sup>channel), as well as ouabain (Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase inhibitor) did not affect hypoxia-induced relaxation. The relaxation was enhanced in the presence of the voltage-gated K<jats:sup>+<\/jats:sup>channel blocker 4-aminopyridine. In conclusion, our experiments suggest that the mETC plays a critical role in O<jats:sub>2<\/jats:sub>sensing in fetal chicken femoral arteries. In contrast, hypoxia-induced relaxation appears not to be mediated by ROS or K<jats:sup>+<\/jats:sup>channels.<\/jats:p>","DOI":"10.1152\/ajpregu.00500.2009","type":"journal-article","created":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T02:55:06Z","timestamp":1264042506000},"page":"R1026-R1034","source":"Crossref","is-referenced-by-count":18,"title":["Hypoxia sensing in the fetal chicken femoral artery is mediated by the mitochondrial electron transport chain"],"prefix":"10.1152","volume":"298","author":[{"given":"Bea","family":"Zoer","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Maastricht University Medical Centre, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The Netherlands;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel L.","family":"Cogolludo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Universidad Complutense Madrid and Centro de Investigaciones Biomedicas en Red de Enfermedades Respiratorias, Madrid, Spain; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Perez-Vizcaino","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Universidad Complutense Madrid and Centro de Investigaciones Biomedicas en Red de Enfermedades Respiratorias, Madrid, Spain; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo G. R.","family":"De Mey","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos E.","family":"Blanco","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Maastricht University Medical Centre, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The Netherlands;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Villamor","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Maastricht University Medical Centre, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The Netherlands;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00204.2006"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.278.4.R980"},{"key":"B3","first-page":"131","volume":"17","author":"Archer S","year":"2002","journal-title":"News Physiol Sci"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000137173.42723.fb"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2006.07.008"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005427919188"},{"key":"B7","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.1242\/jeb.204.18.3171","volume":"204","author":"Boutilier RG","year":"2001","journal-title":"J Exp Biol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn349"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00092.2009"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020565"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4715"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1994.267.2.H706"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00316.2008"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00817.2009"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00001.2008"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00629.2004"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00615.2005"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2007.1995"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00909.2006"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00320-8"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.649566"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00211.x"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00337.2002"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.280.5.L856"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02452-1"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000024689.07590.C2"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000035057.63303.D1"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1996.270.3.H1044"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2009.06.009"},{"key":"B30","first-page":"145","volume":"9","author":"Murad F","year":"1978","journal-title":"Adv Cyclic Nucleotide Res"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02480"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.280.1.R198"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00149.2002"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00683.2003"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01107.2005"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.10.116"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0253b.x"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00675.2001"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00107.2002"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000055770.07236.98"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.102.23.2892"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1002\/jez.427"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.281.5.R1506"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.8.862"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207217200"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4409"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00654.2006"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(83)90065-8"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318194fa8f"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00611.2003"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00369.2001"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2007.10.010"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00480.2006"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000036751.04896.F1"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00722.2004"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2007.041236"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12147.x"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050002"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00480a.2006"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2002-0217OC"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00376.2002"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01167.2008"},{"key":"B63","first-page":"119","volume":"60","author":"Zoer B","year":"2009","journal-title":"J Physiol Pharmacol"}],"container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpregu.00500.2009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T15:40:25Z","timestamp":1634744425000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpregu.00500.2009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":63,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1152\/ajpregu.00500.2009"],"URL":"https:\/\/doi.org\/10.1152\/ajpregu.00500.2009","relation":{},"ISSN":["0363-6119","1522-1490"],"issn-type":[{"value":"0363-6119","type":"print"},{"value":"1522-1490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4]]}}}