{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:11:39Z","timestamp":1790835099545,"version":"4.1.0"},"reference-count":55,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2014,9,15]]},"abstract":"<jats:p>Guanylin (GN) inhibited water absorption and short-circuit current ( I<jats:sub>sc<\/jats:sub>) in seawater eel intestine. Similar inhibition was observed after bumetanide, and the effect of bumetanide was abolished by GN or vice versa, suggesting that both act on the same target, Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter (NKCC), which is a key player for the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>transport system responsible for water absorption in marine teleost intestine. However, effect of GN was always greater than that of bumetanide: 10% greater in middle intestine (MI) and 40% in posterior intestine (PI) for I<jats:sub>sc<\/jats:sub>, and 25% greater in MI and 34% in PI for water absorption. After treatment with GN, I<jats:sub>sc<\/jats:sub>decreased to zero, but 20\u201330% water absorption still remained. The remainder may be due to the Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>exchanger and Na<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>cotransporter (NCC), since inhibitors for these transporters almost nullified the remaining water absorption. Quantitative PCR analysis revealed the presence of major proteins involved in water absorption; the NKCC2\u03b2 and AQP1 genes whose expression was markedly upregulated after seawater acclimation. The SLC26A6 (anion exchanger) and NCC\u03b2 genes were also expressed in small amounts. Consistent with the inhibitors' effect, expression of NKCC2\u03b2 was MI &gt; PI, and that of NCC\u03b2 was MI &lt;&lt; PI. The present study showed that GN not only inhibits the bumetanide-sensitive Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>transport system governed by NKCC2\u03b2, but also regulates unknown ion transporters different from GN-insensitive SLC26A6 and NCC. A candidate is cystic fibrosis transmembrane conductance regulator Cl<jats:sup>\u2212<\/jats:sup>channel, as demonstrated in mammals, but its expression is low in eel intestine, and its role may be minor, as indicated by the small effect of its inhibitors.<\/jats:p>","DOI":"10.1152\/ajpregu.00543.2013","type":"journal-article","created":{"date-parts":[[2014,7,3]],"date-time":"2014-07-03T08:01:19Z","timestamp":1404374479000},"page":"R653-R663","source":"Crossref","is-referenced-by-count":14,"title":["Mechanisms of guanylin action on water and ion absorption at different regions of seawater eel intestine"],"prefix":"10.1152","volume":"307","author":[{"given":"Masaaki","family":"Ando","sequence":"first","affiliation":[{"name":"Laboratory of Physiology, Department of Marine Bioscience, Atmosphere and Ocean Research Institute, The University of Tokyo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marty K. 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