{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T13:56:01Z","timestamp":1789826161869,"version":"4.0.1"},"reference-count":59,"publisher":"American Physiological Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2014,10,1]]},"abstract":"<jats:p>Rhodnius prolixus is a hematophagous insect vector of Chagas disease capable of ingesting up to 10 times its unfed body weight in blood in a single meal. The excess water and ions ingested with the meal are expelled through a rapid postprandial diuresis driven by the Malpighian tubules. Diuresis is triggered by at least two diuretic hormones, a CRF-related peptide and serotonin, which were traditionally believed to trigger cAMP as an intracellular second messenger. Recently, calcium has been suggested to act as a second messenger in serotonin-stimulated Malpighian tubules. Thus, we tested the role of calcium in serotonin-stimulated Malpighian tubules from R. prolixus. Our results show that serotonin triggers cAMP-mediated intracellular Ca<jats:sup>2+<\/jats:sup>waves that were blocked by incubation in Ca<jats:sup>2+<\/jats:sup>-free saline containing the cell membrane-permeant Ca<jats:sup>2+<\/jats:sup>chelator BAPTA-AM, or the PKA blocker H-89. Treatment with 8-Br-cAMP triggered Ca<jats:sup>2+<\/jats:sup>waves that were blocked by H-89 and BAPTA-AM. Analysis of the secreted fluid in BAPTA-AM-treated tubules showed a 75% reduction in fluid secretion rate with increased K<jats:sup>+<\/jats:sup>concentration, reduced Na<jats:sup>+<\/jats:sup>concentration. Taken together, the results indicate that serotonin triggers cAMP and PKA-mediated Ca<jats:sup>2+<\/jats:sup>waves that are required for maximal ion transport rate.<\/jats:p>","DOI":"10.1152\/ajpregu.00561.2013","type":"journal-article","created":{"date-parts":[[2014,7,10]],"date-time":"2014-07-10T03:58:50Z","timestamp":1404964730000},"page":"R828-R836","source":"Crossref","is-referenced-by-count":27,"title":["Serotonin triggers cAMP and PKA-mediated intracellular calcium waves in Malpighian tubules of<i>Rhodnius prolixu<\/i>s"],"prefix":"10.1152","volume":"307","author":[{"given":"Paula","family":"Gioino","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan G.","family":"Murray","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan P.","family":"Ianowski","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","author":"Allen CU","journal-title":"J. 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