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The resting membrane potential of human acinar cells was near \u221251 mV, while the intracellular [Cl<jats:sup>\u2212<\/jats:sup>] was \u223c62 mM, about fourfold higher than expected if Cl ions were passively distributed. Active Cl<jats:sup>\u2212<\/jats:sup>uptake mechanisms included a bumetanide-sensitive Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter and paired DIDS-sensitive Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>and EIPA-sensitive Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>exchangers that correlated with expression of NKCC1, AE2, and NHE1 transcripts, respectively. Intracellular Ca<jats:sup>2+<\/jats:sup>stimulated a niflumic acid-sensitive Cl<jats:sup>\u2212<\/jats:sup>current with properties similar to the Ca<jats:sup>2+<\/jats:sup>-gated Cl channel BEST2. In addition, intracellular Ca<jats:sup>2+<\/jats:sup>stimulated a paxilline-sensitive and voltage-dependent, large-conductance K channel and a clotrimazole-sensitive, intermediate-conductance K channel, consistent with the detection of transcripts for KCNMA1 and KCNN4, respectively. Our results demonstrate that the ion transport mechanisms in human parotid glands are equivalent to those in the mouse, confirming that animal models provide valuable systems for testing therapies to prevent salivary gland dysfunction.<\/jats:p>","DOI":"10.1152\/ajpregu.00591.2006","type":"journal-article","created":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T01:59:17Z","timestamp":1173405557000},"page":"R2380-R2390","source":"Crossref","is-referenced-by-count":47,"title":["Functional and molecular characterization of the fluid secretion mechanism in human parotid acinar cells"],"prefix":"10.1152","volume":"292","author":[{"given":"Tetsuji","family":"Nakamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaka","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor G.","family":"Romanenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine E.","family":"Ovitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Perez-Cornejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Arreola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ted","family":"Begenisich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Melvin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.108.1.35"},{"key":"R2","unstructured":"Atkinson JC, Baum BJ.Salivary enhancement: current status and future therapies.J Dent Educ65: 1096\u20131101, 2001."},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409627200"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406201200"},{"key":"R5","unstructured":"Cook DI, Van Lennep EW, Roberts ML, Young JA.Secretion by the Major Salivary Glands. 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