{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:31:06Z","timestamp":1759336266362},"reference-count":74,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p> The interaction between ghrelin and bombesin or amylin administered intraperitoneally on food intake and brain neuronal activity was assessed by Fos-like immunoreactivity (FLI) in nonfasted rats. Ghrelin (13 \u03bcg\/kg ip) increased food intake compared with the vehicle group when measured at 30 min (g\/kg: 3.66 \u00b1 0.80 vs. 1.68 \u00b1 0.42, P &lt; 0.0087). Bombesin (8 \u03bcg\/kg) injected intraperitoneally with ghrelin (13 \u03bcg\/kg) blocked the orexigenic effect of ghrelin (1.18 \u00b1 0.41 g\/kg, P &lt; 0.0002). Bombesin alone (4 and 8 \u03bcg\/kg ip) exerted a dose-related nonsignificant reduction of food intake (g\/kg: 1.08 \u00b1 0.44, P &gt; 0.45 and 0.55 \u00b1 0.34, P &gt; 0.16, respectively). By contrast, ghrelin-induced stimulation of food intake (g\/kg: 3.96 \u00b1 0.56 g\/kg vs. vehicle 0.82 \u00b1 0.59, P &lt; 0.004) was not altered by amylin (1 and 5 \u03bcg\/kg ip) (g\/kg: 4.37 \u00b1 1.12, P &gt; 0.69, and 3.01 \u00b1 0.78, respectively, P &gt; 0.37). Ghrelin increased the number of FLI-positive neurons\/section in the arcuate nucleus (ARC) compared with vehicle (median: 42 vs. 19, P &lt; 0.008). Bombesin alone (4 and 8 \u03bcg\/kg ip) did not induce FLI neurons in the paraventricular nucleus of the hypothalamus (PVN) and coadministered with ghrelin did not alter ghrelin-induced FLI in the ARC. However, bombesin (8 \u03bcg\/kg) with ghrelin significantly increased neuronal activity in the PVN approximately threefold compared with vehicle and \u223c1.5-fold compared with the ghrelin group. Bombesin (8 \u03bcg\/kg) with ghrelin injected intraperitoneally induced Fos expression in 22.4 \u00b1 0.8% of CRF-immunoreactive neurons in the PVN. These results suggest that peripheral bombesin, unlike amylin, inhibits peripheral ghrelin induced food intake and enhances activation of CRF neurons in the PVN. <\/jats:p>","DOI":"10.1152\/ajpregu.00681.2005","type":"journal-article","created":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T20:49:38Z","timestamp":1146170978000},"page":"R903-R913","source":"Crossref","is-referenced-by-count":16,"title":["Bombesin, but not amylin, blocks the orexigenic effect of peripheral 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