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We used this new intronic OT probe, together with other well-established intronic and exonic OT and VP probes, to reevaluate OT and VP gene expression in the hypothalamus under two classical physiological conditions, acute osmotic stimulation, and lactation. We found that magnocellular neurons in 7- to 8-day lactating female rats exhibit increased OT but not VP hnRNA. Since VP mRNA is increased during lactation, this suggests that decreased VP mRNA degradation during lactation may be responsible for this change. In contrast, whereas there was the expected large increase in VP hnRNA after acute salt loading, there was no change in OT hnRNA, suggesting that acute hyperosmotic stimuli produce increased VP but not OT gene transcription. Hence, the use of both exon- and intron-specific probes, which distinguish the changes in hnRNA and mRNA levels, respectively, can provide insight into the relative roles of transcription and mRNA degradation processes in changes in gene expression evoked by physiological stimuli.<\/jats:p>","DOI":"10.1152\/ajpregu.00709.2005","type":"journal-article","created":{"date-parts":[[2005,12,16]],"date-time":"2005-12-16T01:33:48Z","timestamp":1134696828000},"page":"R1233-R1241","source":"Crossref","is-referenced-by-count":21,"title":["Studies of oxytocin and vasopressin gene expression in the rat hypothalamus using exon- and intron-specific probes"],"prefix":"10.1152","volume":"290","author":[{"given":"Chunmei","family":"Yue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Mutsuga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elka 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