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RT-PCR revealed NHE2 transcripts predominantly in gill, stomach, rectal gland, intestine, and kidney. In situ hybridization with an antisense probe against NHE2 in gill sections revealed a strong mRNA signal from a subset of interlamellar and lamellae cells. We developed dogfish-specific polyclonal antibodies against NHE2 that detected a \u223c70-kDa protein in Western blots and immunologically recognized branchial cells having two patterns of protein expression. Cytoplasmic and apical NHE2 immunoreactivity were observed in cells coexpressing basolateral Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase. Other large ovoid cells more generally staining for NHE2 also were strongly positive for basolateral H<jats:sup>+<\/jats:sup>-ATPase. Gill mRNA levels for NHE2 and H<jats:sup>+<\/jats:sup>-ATPase did not change following systemic acidosis (as measured by quantitative PCR 2 h after a 1- or 2-meq\/kg acid infusion). These data indicate that posttranslational adjustments of NHE2 and other transport systems (e.g., NHE3) following acidosis may be of importance in the short-term pH adjustment and net branchial H<jats:sup>+<\/jats:sup>efflux observed in vivo. NHE2 may play multiple roles in the gills, involved with H<jats:sup>+<\/jats:sup>efflux from acid-secreting cells, basolateral H<jats:sup>+<\/jats:sup>reabsorption for pH<jats:sub>i<\/jats:sub>regulation, and in parallel with H<jats:sup>+<\/jats:sup>-ATPase for the generation of HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>in base-secreting cells.<\/jats:p>","DOI":"10.1152\/ajpregu.00718.2007","type":"journal-article","created":{"date-parts":[[2007,12,20]],"date-time":"2007-12-20T01:44:31Z","timestamp":1198115071000},"page":"R1092-R1102","source":"Crossref","is-referenced-by-count":21,"title":["Molecular detection and immunological localization of gill Na<sup>+<\/sup>\/H<sup>+<\/sup>exchanger in the dogfish (<i>Squalus acanthias<\/i>)"],"prefix":"10.1152","volume":"294","author":[{"given":"James B.","family":"Claiborne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith P.","family":"Choe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison I.","family":"Morrison-Shetlar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill C.","family":"Weakley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Havird","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abe","family":"Freiji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan L.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Argos P, Rao JK, Hargrave PA.Structural prediction of membrane-bound proteins.Eur J Biochem128: 565\u2013575, 1982.","DOI":"10.1111\/j.1432-1033.1982.tb07002.x"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.02.014"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.074716"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.5.C1496"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00360.2004"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02384"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.6.C1111"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.6.C2039"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.281.4.F707"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2006.09.027"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(99)00108-7"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00048.2005"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(01)00491-3"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00513.2003"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.15.10481"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh114"},{"key":"R17","unstructured":"Claiborne JB.Acid-Base Regulation. 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