{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:11:45Z","timestamp":1790835105994,"version":"4.1.0"},"reference-count":68,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>Intestinal HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>secretion and NaCl absorption are essential for counteracting dehydration in marine teleost fish. We investigated how these two processes are coordinated in toadfish. HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>stimulated a luminal positive short-circuit current ( I<jats:sub>sc<\/jats:sub>) in intestine mounted in Ussing chamber, bathed with the same saline solution on the external and internal sides of the epithelium. The I<jats:sub>sc<\/jats:sub>increased proportionally to the [HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>] in the bath up to 80 mM NaHCO<jats:sub>3<\/jats:sub>, and it did not occur when NaHCO<jats:sub>3<\/jats:sub>was replaced with Na<jats:sup>+<\/jats:sup>-gluconate or with NaHCO<jats:sub>3<\/jats:sub>in Cl<jats:sup>\u2212<\/jats:sup>-free saline. HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>(20 mM) induced a \u223c2.5-fold stimulation of I<jats:sub>sc<\/jats:sub>, and this [HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>] was used in all subsequent experiments. The HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-stimulated I<jats:sub>sc<\/jats:sub>was prevented or abolished by apical application of 10 \u03bcM bumetanide (a specific inhibitor of NKCC) and by 30 \u03bcM 4-catechol estrogen [CE; an inhibitor of soluble adenylyl cyclase (sAC)]. The inhibitory effects of bumetanide and CE were not additive. The HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-stimulated I<jats:sub>sc<\/jats:sub>was prevented by apical bafilomycin (1 \u03bcM) and etoxolamide (1 mM), indicating involvement of V-H<jats:sup>+<\/jats:sup>-ATPase and carbonic anhydrases, respectively. Immunohistochemistry and Western blot analysis confirmed the presence of an NKCC2-like protein in the apical membrane and subapical area of epithelial intestinal cells, of Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase in basolateral membranes, and of an sAC-like protein in the cytoplasm. We propose that sAC regulates NKCC activity in response to luminal HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>, and that V-H<jats:sup>+<\/jats:sup>-ATPase and intracellular carbonic anhydrase are essential for transducing luminal HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>into the cell by CO<jats:sub>2<\/jats:sub>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>hydration\/dehydration. This mechanism putatively coordinates HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>secretion with NaCl and water absorption in toadfish intestine.<\/jats:p>","DOI":"10.1152\/ajpregu.00761.2009","type":"journal-article","created":{"date-parts":[[2010,4,22]],"date-time":"2010-04-22T03:07:55Z","timestamp":1271905675000},"page":"R62-R71","source":"Crossref","is-referenced-by-count":54,"title":["Modulation of NaCl absorption by [HCO<sub>3<\/sub><sup>\u2212<\/sup>] in the marine teleost intestine is mediated by soluble adenylyl cyclase"],"prefix":"10.1152","volume":"299","author":[{"given":"Martin","family":"Tresguerres","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Weill Cornell Medical College, New York, New York; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lonny R.","family":"Levin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Weill Cornell Medical College, New York, New York; 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