{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:31:23Z","timestamp":1790800283282,"version":"4.1.0"},"reference-count":38,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:p>We investigated the involvement of carbonic anhydrase (CA) in mediating V-H<jats:sup>+<\/jats:sup>-ATPase translocation into the basolateral membrane in gills of alkalotic Squalus acanthias. Immunolabeling revealed that CA is localized in the same cells as V-H<jats:sup>+<\/jats:sup>-ATPase. Blood plasma from dogfish injected with acetazolamide [30 mg\/kg at time ( t) = 0 and 6 h] and infused with NaHCO<jats:sub>3<\/jats:sub>for 12 h (1,000 \u03bceq\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7h<jats:sup>\u22121<\/jats:sup>) had significantly higher plasma HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>concentration than fish that were infused with NaHCO<jats:sub>3<\/jats:sub>alone (28.72 \u00b1 0.41 vs. 6.57 \u00b1 2.47 mmol\/l, n = 3), whereas blood pH was similar in both treatments (8.03 \u00b1 0.11 vs. 8.04 \u00b1 0.11 pH units at t = 12 h). CA inhibition impaired V-H<jats:sup>+<\/jats:sup>-ATPase translocation into the basolateral membrane, as estimated from immunolabeled gill sections and Western blotting on gill cell membranes (0.24 \u00b1 0.08 vs. 1.00 \u00b1 0.28 arbitrary units, n = 3; P &lt; 0.05). We investigated V-H<jats:sup>+<\/jats:sup>-ATPase translocation during a postfeeding alkalosis (\u201calkaline tide\u201d). Gill samples were taken 24\u201326 h after dogfish were fed to satiety in a natural-like feeding regime. Immunolabeled gill sections revealed that V-H<jats:sup>+<\/jats:sup>-ATPase translocated to the basolateral membrane in the postfed fish. Confirming this result, V-H<jats:sup>+<\/jats:sup>-ATPase abundance was twofold higher in gill cell membranes of the postfed fish than in fasted fish ( n = 4\u20135; P &lt; 0.05). These results indicate that 1) intracellular H<jats:sup>+<\/jats:sup>or HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>produced by CA (and not blood pH or HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>) is likely the stimulus that triggers the V-H<jats:sup>+<\/jats:sup>-ATPase translocation into the basolateral membrane in alkalotic fish and 2) V-H<jats:sup>+<\/jats:sup>-ATPase translocation is important for enhanced HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>secretion during a naturally occurring postfeeding alkalosis.<\/jats:p>","DOI":"10.1152\/ajpregu.00814.2006","type":"journal-article","created":{"date-parts":[[2007,1,5]],"date-time":"2007-01-05T01:59:30Z","timestamp":1167962370000},"page":"R2012-R2019","source":"Crossref","is-referenced-by-count":45,"title":["V-H<sup>+<\/sup>-ATPase translocation during blood alkalosis in dogfish gills: interaction with carbonic anhydrase and involvement in the postfeeding alkaline tide"],"prefix":"10.1152","volume":"292","author":[{"given":"Martin","family":"Tresguerres","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott K.","family":"Parks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris M.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg G.","family":"Goss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2005.10.001"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.3.F437"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412750200"},{"key":"R4","unstructured":"Boutilier RG, Heming TA, Iwama GK.Appendix: physicochemical parameters for use in fish respiratory physiology. 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