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CYP-induced cystitis significantly ( P \u2264 0.01) increased pERK expression in the urinary bladder with intermediate (48 h) and chronic CYP treatment. Immunohistochemistry for pERK immunoreactivity revealed little pERK-IR in control or acute (4 h) CYP-treated rat urinary bladders. However, pERK expression was significantly ( P \u2264 0.01) upregulated in the urothelium after 48 h or chronic CYP treatment. Whole mount preparations of urothelium\/lamina propria or detrusor smooth muscle from control (noninflamed) rats showed no pERK-IR in PGP9.5-labeled nerve fibers in the suburothelial plexus. However, with CYP-treatment (48 h, chronic), a few pERK-IR nerve fibers in the suburothelial plexus of whole mount preparations of bladder and at the serosal edge of urinary bladder sections were observed. pERK-IR cells expressing the CD86 antigen were also observed in urinary bladder from CYP-treated rats (48 h, chronic). Treatment with the upstream inhibitor of ERK phosphorylation, U0126, significantly ( P \u2264 0.01) increased bladder capacity in CYP-treated rats (48 h). These studies suggest that therapies targeted at pERK pathways may improve urinary bladder function in CYP-treated rats.<\/jats:p>","DOI":"10.1152\/ajpregu.00857.2006","type":"journal-article","created":{"date-parts":[[2007,4,4]],"date-time":"2007-04-04T20:35:00Z","timestamp":1175718900000},"page":"R125-R134","source":"Crossref","is-referenced-by-count":43,"title":["Phosphorylation of extracellular signal-regulated kinases in urinary bladder in rats with cyclophosphamide-induced cystitis"],"prefix":"10.1152","volume":"293","author":[{"given":"Kimberly A.","family":"Corrow","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret A.","family":"Vizzard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2004.09.016"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00038.2003"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00496-1"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-02-00859.1999"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00287.2006"},{"key":"R6","unstructured":"Braas KM\n                      , May V, Zvara P, Nausch B, Kliment J, Dunleavy JD, Nelson MT.\n                      Role for pituitary adenylate cyclase activating polypeptide (PACAP) in cystitis-induced plasticity of micturition reflexes.\n                      Regul Pept\n                      130\n                      : 157\u2013158, 2005."},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00734.2005"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2003.10.034"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2005.03893.x"},{"key":"R10","unstructured":"Damoiseaux JGMC\n                      , Dopp EA, Calame W, Chao D, MacPherson GG.\n                      Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1.\n                      Immunology\n                      83\n                      : 140\u2013147, 1994."},{"key":"R11","unstructured":"Dinarello CA\n                      .\n                      Overview of inflammatory cytokines and their role in pain. 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