{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,7,9]],"date-time":"2022-07-09T14:51:44Z","timestamp":1657378304280},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[1996,9,1]]},"abstract":"<jats:p> Stimulation of vasopressin (VP) gene expression by adenosine 3',5'-cyclic monophosphate (cAMP) has been observed in dispersed hypothalamic cultures, in VP-expressing cell lines, and in cells transfected with reporter genes regulated by the VP gene promoter. However, treatment of hypothalamo-neurohypophysial system (HNS) explants with forskolin (25 microM), an activator of adenyl cyclase, and 3-isobutyl-1-methylxanthine (IBMX; 500 microM), a phosphodiesterase inhibitor, resulted in a decrease in VP mRNA. Time course analysis revealed that IBMX and forskolin reduced the VP mRNA content to 50% of control explants after 8 and 12 h despite a dramatic stimulation of VP release. This effect was due to the activation of adenyl cyclase by forskolin, because neither IBMX alone nor the inactive analogue of forskolin, 1,9-dideoxyforskolin, decreased VP mRNA content. In contrast, 8-bromoadenosine 3',5'-cyclic monophosphate and the D1 dopamine receptor agonist, SKF-38393, increased VP mRNA content, but these agents were less potent in stimulating VP release, suggesting a concentration dependency of the forskolin effect. This was confirmed when forskolin (10 microM) was found to increase VP mRNA content. Thus receptor-mediated activation of adenyl cyclase results in an increase in VP mRNA content. <\/jats:p>","DOI":"10.1152\/ajpregu.1996.271.3.r554","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T23:47:16Z","timestamp":1514159236000},"page":"R554-R560","source":"Crossref","is-referenced-by-count":2,"title":["cAMP regulation of vasopressin mRNA content in hypothalamo-neurohypophysial explants"],"prefix":"10.1152","volume":"271","author":[{"given":"C. D.","family":"Sladek","sequence":"first","affiliation":[{"name":"Department of Physiology, Finch University of Health Sciences, ChicagoMedical School, Illinois 60064, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Y.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Physiology, Finch University of Health Sciences, ChicagoMedical School, Illinois 60064, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. E.","family":"Sidorowicz","sequence":"additional","affiliation":[{"name":"Department of Physiology, Finch University of Health Sciences, ChicagoMedical School, Illinois 60064, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Mathiasen","sequence":"additional","affiliation":[{"name":"Department of Physiology, Finch University of Health Sciences, ChicagoMedical School, Illinois 60064, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpregu.1996.271.3.R554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T03:24:50Z","timestamp":1567999490000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpregu.1996.271.3.R554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,9,1]]}},"alternative-id":["10.1152\/ajpregu.1996.271.3.R554"],"URL":"https:\/\/doi.org\/10.1152\/ajpregu.1996.271.3.r554","relation":{},"ISSN":["0363-6119","1522-1490"],"issn-type":[{"value":"0363-6119","type":"print"},{"value":"1522-1490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9,1]]}}}