{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T05:20:23Z","timestamp":1782883223525,"version":"3.54.5"},"reference-count":32,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2001,1,1]]},"abstract":"<jats:p> TGR(ASrAOGEN)680, a newly developed transgenic rat line with specific downregulation of astroglial synthesis of angiotensinogen, exhibits decreased brain angiotensinogen content associated with a mild diabetes insipidus and lower blood pressure. Autoradiographic experiments were performed on TGR(ASrAOGEN) (TG) and Sprague-Dawley (SD) control rats to quantify AT<jats:sub>1<\/jats:sub> and AT<jats:sub>2<\/jats:sub> receptor-binding sites in different brain nuclei and circumventricular organs. Dose-response curves for drinking response to intracerebroventricular injections of ANG II were compared between SD and TG rats. <\/jats:p><jats:p> In most of the regions inside the blood-brain barrier [paraventricular nucleus (PVN), piriform cortex, lateral olfactory tract (LOT), and lateral preoptic area (LPO)], AT<jats:sub>1<\/jats:sub> receptor binding (sensitive to CV-11974) was significantly higher in TG compared with SD. In contrast, in the circumventricular organs investigated [subfornical organ (SFO) and area postrema], AT<jats:sub>1<\/jats:sub> receptor binding was significantly lower in TG. AT<jats:sub>2<\/jats:sub> receptors (binding sensitive to PD-123319) were detected at similar levels in the inferior olive (IO) of both strains. Angiotensin-binding sites sensitive to both CV-11974 and PD-123319 were detected in the LPO of SD rats and specifically upregulated in LOT, IO, and most notably PVN and SFO of TG. The dose-response curve for water intake after intracerebroventricular injections showed a higher sensitivity to ANG II of TG (EC<jats:sub>50<\/jats:sub> = 3.1 ng) compared with SD (EC<jats:sub>50<\/jats:sub> = 11.2 ng), strongly suggesting that the upregulation of AT<jats:sub>1<\/jats:sub> receptors inside the blood-brain barrier of TG rats is functional. <\/jats:p><jats:p> Finally, we showed that downregulation of angiotensinogen synthesized by astroglial cells differentially regulates angiotensin receptor subtypes inside the brain and in circumventricular organs. <\/jats:p>","DOI":"10.1152\/ajpregu.2001.280.1.r233","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T05:08:37Z","timestamp":1514178517000},"page":"R233-R240","source":"Crossref","is-referenced-by-count":39,"title":["Glial angiotensinogen regulates brain angiotensin II receptors in transgenic rats TGR(ASrAOGEN)"],"prefix":"10.1152","volume":"280","author":[{"given":"Jan","family":"Monti","sequence":"first","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine, 13092 Berlin;"},{"name":"Institut National de la Sant\u00e9et de la Recherche M\u00e9dicale Unit\u00e9 331, 69675 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Schinke","sequence":"additional","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine, 13092 Berlin;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"B\u00f6hm","sequence":"additional","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine, 13092 Berlin;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Detlev","family":"Ganten","sequence":"additional","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine, 13092 Berlin;"},{"name":"Department of Clinical Pharmacology, University Hospital Benjamin Franklin, Free University Berlin, 12200 Berlin, Germany; 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