{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:16:27Z","timestamp":1780668987000,"version":"3.54.1"},"reference-count":39,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2001,4,1]]},"abstract":"<jats:p>We hypothesized that nitric oxide (NO) contributes to intrasplenic fluid extravasation by inducing greater relaxation in splenic resistance arteries than veins such that intrasplenic microvascular pressure (P<jats:sub>C<\/jats:sub>) rises. Fluid efflux was estimated by measuring the difference between splenic blood inflow and outflow. Intrasplenic infusion of the NO donor S-nitroso- N-acetylpenicillamine (SNAP) (0.3 \u03bcg \u00b7 10 \u03bcl<jats:sup>\u22121<\/jats:sup>\u00b7 min<jats:sup>\u22121<\/jats:sup>) caused a significant increase in intrasplenic fluid efflux (baseline: 0.8 \u00b1 0.4 ml\/min, n = 10 vs. peak rise during SNAP infusion: 1.3 \u00b1 0.4 ml\/min, n = 10; P &lt; 0.05). Intrasplenic P<jats:sub>C<\/jats:sub>was measured in the isolated, blood-perfused rat spleen. Intrasplenic infusion of SNAP (0.1 \u03bcg \u00b7 10 \u03bcl<jats:sup>\u22121<\/jats:sup>\u00b7 min<jats:sup>\u22121<\/jats:sup>) caused a significant increase in P<jats:sub>C<\/jats:sub>(saline: 10.9 \u00b1 0.2 mmHg, n = 3 vs. SNAP: 12.2 \u00b1 0.2 mmHg, n = 3; P &lt; 0.05). Vasoreactivity of preconstricted splenic resistance vessels to sodium nitroprusside (SNP) (1 \u00d7 10<jats:sup>\u221212<\/jats:sup>-1 \u00d7 10<jats:sup>\u22124<\/jats:sup>M) and SNAP (1 \u00d7 10<jats:sup>\u221210<\/jats:sup>-3 \u00d7 10<jats:sup>\u22124<\/jats:sup>M) was investigated with the use of a wire myograph system. Significantly greater relaxation of arterioles than of venules occurred with both SNP (%maximal vasorelaxation: artery 96 \u00b1 2.3, n = 9 vs. vein 26 \u00b1 1.9, n = 10) and SNAP (%maximal vasorelaxation: artery 50 \u00b1 3.5, n = 11 vs. vein 32 \u00b1 1.7, n = 8). These results are consistent with our proposal that differential vasoreactivity of splenic resistance arteries and veins to NO elevates intrasplenic P<jats:sub>C<\/jats:sub>and increases fluid extravasation into the systemic lymphatic system.<\/jats:p>","DOI":"10.1152\/ajpregu.2001.280.4.r959","type":"journal-article","created":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T05:28:54Z","timestamp":1514179734000},"page":"R959-R967","source":"Crossref","is-referenced-by-count":15,"title":["Nitric oxide increases fluid extravasation from the splenic circulation of the rat"],"prefix":"10.1152","volume":"280","author":[{"given":"Peter S.","family":"Andrew","sequence":"first","affiliation":[{"name":"Departments of Physiology and Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Deng","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Sultanian","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Kaufman","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, 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