{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:53:43Z","timestamp":1773420823287,"version":"3.50.1"},"reference-count":35,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:p> Activation of neuronal ATP-sensitive potassium (K<jats:sub>ATP<\/jats:sub>) channels is an important mechanism that protects neurons and conserves neural function during hypoxia. We investigated hypoxia (bath gassed with 95% N<jats:sub>2<\/jats:sub>-5% CO<jats:sub>2<\/jats:sub> vs. 95% O<jats:sub>2<\/jats:sub>-5% CO<jats:sub>2<\/jats:sub> in control)-induced changes in K<jats:sub>ATP<\/jats:sub> current in second-order neurons of peripheral chemoreceptors in the nucleus of the solitary tract (NTS). Hypoxia-induced K<jats:sub>ATP<\/jats:sub> currents were compared between normoxic (Norm) rats and rats exposed to 1 wk of either chronic sustained hypoxia (CSH) or chronic intermittent hypoxia (CIH). Whole cell recordings of NTS second-order neurons identified after 4-(4-(dihexadecylamino)styryl)- N-methylpyridinium iodide (DiA) labeling of the carotid bodies were obtained in a brain stem slice. In Norm cells ( n = 9), hypoxia (3 min) induced an outward current of 12.7 \u00b1 1.1 pA with a reversal potential of \u221273 \u00b1 2 mV. This current was completely blocked by the K<jats:sub>ATP<\/jats:sub> channel blocker tolbutamide (100 \u03bcM). Bath application of the K<jats:sub>ATP<\/jats:sub> channel opener diazoxide (200 \u03bcM, 3 min) evoked an outward current of 21.8 \u00b1 5.8 pA ( n = 6). Hypoxia elicited a significantly smaller outward current in both CSH (5.9 \u00b1 1.4 pA, n = 11; P &lt; 0.01) and CIH (6.8 \u00b1 1.7 pA, n = 6; P &lt; 0.05) neurons. Diazoxide elicited a significantly smaller outward current in CSH (3.9 \u00b1 1.0 pA, n = 5; P &lt; 0.05) and CIH (2.9 \u00b1 0.9 pA, n = 3; P &lt; 0.05) neurons. Western blot analysis showed reduced levels of K<jats:sub>ATP<\/jats:sub> potassium channel subunits Kir6.1 and Kir6.2 in the NTS from CSH and CIH rats. These results suggest that hypoxia activates K<jats:sub>ATP<\/jats:sub> channels in NTS neurons receiving monosynaptic chemoreceptor afferent inputs. Chronic exposure to either sustained or intermittent hypoxia reduces K<jats:sub>ATP<\/jats:sub> channel function in NTS neurons. This may represent a neuronal adaptation that preserves neuronal excitability in crucial relay neurons in peripheral chemoreflex pathways. <\/jats:p>","DOI":"10.1152\/ajpregu.90390.2008","type":"journal-article","created":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T21:13:11Z","timestamp":1221081191000},"page":"R1555-R1562","source":"Crossref","is-referenced-by-count":47,"title":["Chronic sustained and intermittent hypoxia reduce function of ATP-sensitive potassium channels in nucleus of the solitary tract"],"prefix":"10.1152","volume":"295","author":[{"given":"Weirong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via R.","family":"Carre\u00f1o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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