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Zebrafish CA IX was highly expressed in the eye, brain, and gastrointestinal tract and showed increased expression in the eye, brain, and muscle in response to hypoxia (water Po<jats:sub>2<\/jats:sub>= 24 mmHg). The hypothesis that increased CA IX expression during hypoxia would act to attenuate intracellular acidosis was then examined. Muscle intracellular pH (pH<jats:sub>i<\/jats:sub>) decreased after 4 h of hypoxic exposure (from 7.15 \u00b1 0.02 to 7.06 \u00b1 0.01 pH units) and did not recover by 24 h. Manipulation of extracellular CA activity via intraperitoneal injection of either bovine CA or the selective extracellular CA inhibitor F3500 revealed that although increased CA activity could fully restore pH<jats:sub>i<\/jats:sub>, removal of extracellular activity did not result in further acidosis. An exercise-induced acidosis was also attenuated in fish treated with bovine CA; however, the increased extracellular CA expression resulting from hypoxia had no affect. These data suggest that although extracellular CA can potentially minimize the impact of hypoxia on muscle pH<jats:sub>i<\/jats:sub>, the actual level of extracellular CA activity is likely insufficient to achieve this goal, even when enhanced by hypoxia-induced increases in CA IX expression.<\/jats:p>","DOI":"10.1152\/ajpregu.90685.2008","type":"journal-article","created":{"date-parts":[[2008,10,23]],"date-time":"2008-10-23T01:11:10Z","timestamp":1224724270000},"page":"R150-R160","source":"Crossref","is-referenced-by-count":21,"title":["Hypoxia-inducible carbonic anhydrase IX expression is insufficient to alleviate intracellular metabolic acidosis in the muscle of zebrafish,<i>Danio rerio<\/i>"],"prefix":"10.1152","volume":"296","author":[{"given":"Andrew J.","family":"Esbaugh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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