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Using laser-scanning confocal microscopy and two models of cell polarization, polycystin-1 and desmosomes were found to colocalize during the initial establishment of cell-cell contact when junctions were forming. However, colocalization was lost in confluent monolayers. Parallel morphological and biochemical evaluations revealed a profound mispolarization of desmosomal components to both the apical and basolateral domains in primary ADPKD cells and tissue. Studies of the intermediate filament network associated with desmosomes showed that there is a decrease in cytokeratin levels and an abnormal expression of the mesenchymal protein vimentin in the disease. Moreover, we show for the first time that the structural alterations seen in adherens and desmosomal junctions have a functional impact, leaving the ADPKD cells with weakened cell-cell adhesion. In conclusion, in this paper we show that polycystin-1 transiently colocalizes with desmosomes and that desmosomal proteins are mislocalized as a consequence of polycystin-1 mutation. <\/jats:p>","DOI":"10.1152\/ajprenal.00008.2005","type":"journal-article","created":{"date-parts":[[2005,2,9]],"date-time":"2005-02-09T04:05:51Z","timestamp":1107921951000},"page":"F1153-F1163","source":"Crossref","is-referenced-by-count":50,"title":["Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease"],"prefix":"10.1152","volume":"288","author":[{"given":"Melina","family":"Silberberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audra J.","family":"Charron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Bacallao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Wandinger-Ness","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12613462"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.52.1.93"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(03)00169-7"},{"key":"R4","unstructured":"Breuning MH. 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