{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T10:29:00Z","timestamp":1785234540460,"version":"3.55.0"},"reference-count":46,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:p>Klotho, a protein counteracting aging, is a powerful inhibitor of 1,25-dihydroxyvitamin D<jats:sub>3<\/jats:sub>[1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>] formation and regulator of mineral metabolism. In klotho hypomorphic ( kl\/kl) mice, excessive 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>formation leads to hypercalcemia, hyperphosphatemia and vascular calcification, severe growth deficits, accelerated aging and early death. Kl\/kl mice further suffer from extracellular volume depletion and hypotension, leading to the stimulation of antidiuretic hormone and aldosterone release. A vitamin D-deficient diet, restriction of dietary phosphate, inhibition of mineralocorticoid receptors with spironolactone, and dietary NaCl all extend the lifespan of kl\/kl mice. Kl\/kl mice suffer from acidosis. The present study explored whether replacement of tap drinking water by 150 mM NaHCO<jats:sub>3<\/jats:sub>affects the growth, tissue calcification, and lifespan of kl\/kl mice. As a result, NaHCO<jats:sub>3<\/jats:sub>administration to kl\/kl mice did not reverse the growth deficit but substantially decreased tissue calcification and significantly increased the average lifespan from 78 to 127 days. NaHCO<jats:sub>3<\/jats:sub>did not significantly affect plasma concentrations of 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>and Ca<jats:sup>2+<\/jats:sup>but significantly decreased plasma phosphate concentration and plasma aldosterone concentration. The present study reveals a novel effect of bicarbonate, i.e., a favorable influence on vascular calcification and early death of klotho-deficient mice.<\/jats:p>","DOI":"10.1152\/ajprenal.00037.2015","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T03:03:36Z","timestamp":1446692616000},"page":"F102-F108","source":"Crossref","is-referenced-by-count":22,"title":["Bicarbonate-sensitive calcification and lifespan of klotho-deficient mice"],"prefix":"10.1152","volume":"310","author":[{"given":"Christina B.","family":"Leibrock","sequence":"first","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen, T\u00fcbingen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Voelkl","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen, T\u00fcbingen, 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