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In principal cells of the tubules, leucokinin-VIII decreased the fractional resistance of the basolateral membrane from 0.733 to 0.518. These effects were reversed by the peritubular Ca<jats:sup>2+<\/jats:sup>-channel blocker nifedipine, suggesting a role of peritubular Ca<jats:sup>2+<\/jats:sup>and basolateral Ca<jats:sup>2+<\/jats:sup>channels in signal transduction. In Ca<jats:sup>2+<\/jats:sup>-free Ringer bath, the effects of leucokinin-VIII were partial and transient but were fully restored after the bath Ca<jats:sup>2+<\/jats:sup>concentration was restored. Increasing intracellular Ca<jats:sup>2+<\/jats:sup>with thapsigargin duplicated the effects of leucokinin-VIII, provided that peritubular Ca<jats:sup>2+<\/jats:sup>was present. The kinetics of the effects of leucokinin-VIII is faster than that of thapsigargin, suggesting the activation of inositol-1,4,5-trisphosphate-receptor channels of intracellular stores. Store depletion may then bring about Ca<jats:sup>2+<\/jats:sup>entry into principal cells via nifedipine-sensitive Ca<jats:sup>2+<\/jats:sup>channels in the basolateral membrane.<\/jats:p>","DOI":"10.1152\/ajprenal.00041.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:35:50Z","timestamp":1425414950000},"page":"F499-F508","source":"Crossref","is-referenced-by-count":44,"title":["Leucokinin activates Ca<sup>2+<\/sup>-dependent signal pathway in principal cells of<i>Aedes aegypti<\/i>Malpighian tubules"],"prefix":"10.1152","volume":"283","author":[{"given":"Ming-Jiun","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, New York 14853"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus W.","family":"Beyenbach","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, New York 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