{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T17:12:04Z","timestamp":1776791524483,"version":"3.51.2"},"reference-count":33,"publisher":"American Physiological Society","issue":"6","funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26830063"],"award-info":[{"award-number":["26830063"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:p>Tensin2 (Tns2), an integrin-linked protein, is enriched in podocytes within the glomerulus. Previous studies have revealed that Tns2-deficient mice exhibit defects of the glomerular basement membrane (GBM) soon after birth in a strain-dependent manner. However, the mechanisms for the onset of defects caused by Tns2 deficiency remains unidentified. Here, we aimed to determine the role of Tns2 using newborn Tns2-deficient mice and murine primary podocytes. Ultrastructural analysis revealed that developing glomeruli during postnatal nephrogenesis exhibited abnormal GBM processing due to ectopic laminin-\u03b1<jats:sub>2<\/jats:sub>accumulation followed by GBM thickening. In addition, analysis of primary podocytes revealed that Tns2 deficiency led to impaired podocyte-GBM interaction and massive expression of laminin-\u03b1<jats:sub>2<\/jats:sub>in podocytes. Our study suggests that weakened podocyte-GBM interaction due to Tns2 deficiency causes increased mechanical stress on podocytes by continuous daily filtration after birth, resulting in stressed podocytes ectopically producing laminin-\u03b1<jats:sub>2<\/jats:sub>, which interrupts GBM processing. We conclude that Tns2 plays important roles in the podocyte-GBM interaction and maintenance of the glomerular filtration barrier.<\/jats:p>","DOI":"10.1152\/ajprenal.00055.2020","type":"journal-article","created":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T11:26:04Z","timestamp":1589196364000},"page":"F1520-F1530","source":"Crossref","is-referenced-by-count":14,"title":["Tensin2 is important for podocyte-glomerular basement membrane interaction and integrity of the glomerular filtration barrier"],"prefix":"10.1152","volume":"318","author":[{"given":"Kozue","family":"Uchio-Yamada","sequence":"first","affiliation":[{"name":"Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Osaka University Graduate School of Medicine, Suita, Osaka, 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