{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:40:54Z","timestamp":1778276454081,"version":"3.51.4"},"reference-count":41,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p>Polycystic kidney growth implies expansion of the vasculature, suggesting that vascular endothelial growth factor (VEGF)-dependent processes play a critical role and that VEGF is a putative therapeutic target. Whether an anti-VEGF antibody improves renal cystic disease has not been determined. We administrated 5 mg\/kg B20.4.1, an anti-VEGF-A antibody, or vehicle intraperitoneally twice weekly to 4-wk-old male normal (+\/+) and cystic (Cy\/+) Han:SPRD rats for 6 wk. Renal function, urinary protein excretion, organ\/body weight ratios, cyst volume, tubular epithelial cell (TEC) proliferation, renal VEGF, hypoxia-inducible factor (HIF)-1\u03b1 and -2\u03b1 expression, renal histology, and kidney hypoxia visualized by [<jats:sup>18<\/jats:sup>F]fluoromisonidazole positron emission tomography were assessed. The treated compared with untreated +\/+ rats had lower TEC proliferation rates, whereas Cy\/+ rats receiving B20.4.1 displayed an increased proximal TEC proliferation rate, causing enhanced cyst and kidney growth. The +\/+ and Cy\/+ rats receiving B20.4.1 had severe renal failure and extensive glomerular damage. Proteinuria, which was highest in anti-VEGF-treated Cy\/+ and lowest in untreated normal littermates, was positively correlated with renal HIF-1\u03b1 and negatively correlated with VEGF expression. The untreated Cy\/+ vs. +\/+ rats had higher overall [<jats:sup>18<\/jats:sup>F]fluoromisonidazole uptake. The +\/+ rats receiving B20.4.1 vs. untreated had increased [<jats:sup>18<\/jats:sup>F]fluoromisonidazole uptake, whereas the uptake was unchanged among treated vs. untreated Cy\/+ animals. In conclusion, B20.4.1 caused an exaggerated cystic response of the proximal tubules in cystic rats and severe kidney injury that was associated with low renal VEGF and high HIF-1\u03b1 levels. Anti-VEGF drug therapy may therefore not be a treatment option for polycystic kidney disease.<\/jats:p>","DOI":"10.1152\/ajprenal.00058.2011","type":"journal-article","created":{"date-parts":[[2011,6,16]],"date-time":"2011-06-16T02:07:37Z","timestamp":1308190057000},"page":"F773-F783","source":"Crossref","is-referenced-by-count":26,"title":["Anti-VEGF antibody treatment accelerates polycystic kidney disease"],"prefix":"10.1152","volume":"301","author":[{"given":"Shagun","family":"Raina","sequence":"first","affiliation":[{"name":"Z\u00fcrich Center for Integrated Human Physiology,"},{"name":"Division of Nephrology, University Hospital Z\u00fcrich, Z\u00fcrich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Honer","sequence":"additional","affiliation":[{"name":"Center for Radiopharmaceutical Sciences, ETH Z\u00fcrich,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie 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