{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:15:43Z","timestamp":1789874143702,"version":"4.0.1"},"reference-count":62,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>\n                    Serum and glucocorticoid-regulated kinase 2 (sgk2) is 80% identical to the kinase domain of sgk1, an important mediator of mineralocorticoid-regulated sodium (Na\n                    <jats:sup>+<\/jats:sup>\n                    ) transport in the distal nephron of the kidney. The expression pattern and role in renal function of sgk2 are virtually uncharacterized. In situ hybridization and immunohistochemistry of rodent kidney coupled with real-time RT-PCR of microdissected rat kidney tubules showed robust sgk2 expression in the proximal straight tubule and thick ascending limb of the loop of Henle. Sgk2 expression was minimal in distal tubule cells with aquaporin-2 immunostaining but significant in proximal tubule cells with Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchanger 3 (NHE3) immunostaining. To ascertain whether mineralocorticoids regulate expression of sgk2 in a manner similar to sgk1, we examined sgk2 mRNA expression in the kidneys of adrenalectomized rats treated with physiological doses of aldosterone together with the glucocorticoid receptor antagonist RU486. Northern blot analysis and in situ hybridization showed that, unlike sgk1, sgk2 expression in the kidney was not altered by aldosterone treatment. Based on the observation that sgk2 is expressed in proximal tubule cells that also express NHE3, we asked whether sgk2 regulates NHE3 activity. We heterologously expressed sgk2 in opossum kidney (OKP) cells and measured Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchange activity by Na\n                    <jats:sup>+<\/jats:sup>\n                    -dependent cell pH recovery. Constitutively active sgk2, but not sgk1, stimulated Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchange activity by &gt;30%. Moreover, the sgk2-mediated increase in Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchange activity correlated with an increase in cell surface expression of NHE3. Together, these results suggest that the pattern of expression, regulation, and role of sgk2 within the mammalian kidney are distinct from sgk1 and that sgk2 may play a previously unrecognized role in the control of transtubular Na\n                    <jats:sup>+<\/jats:sup>\n                    transport through NHE3 in the proximal tubule.\n                  <\/jats:p>","DOI":"10.1152\/ajprenal.00075.2010","type":"journal-article","created":{"date-parts":[[2010,10,6]],"date-time":"2010-10-06T22:33:59Z","timestamp":1286404439000},"page":"F1496-F1506","source":"Crossref","is-referenced-by-count":44,"title":["Expression and role of serum and glucocorticoid-regulated kinase 2 in the regulation of Na\n                    <sup>+<\/sup>\n                    \/H\n                    <sup>+<\/sup>\n                    exchanger 3 in the mammalian kidney"],"prefix":"10.1152","volume":"299","author":[{"given":"Alan C.","family":"Pao","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Stanford University, Stanford;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Bhargava","sequence":"additional","affiliation":[{"name":"Department of Surgery,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca Di","family":"Sole","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine,"},{"name":"Charles and Jane Pak Center of Mineral Metabolism, University of Texas Southwestern Medical Center, Dallas, Texas; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Quigley","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Pediatrics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinli","family":"Shao","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheela","family":"Thomas","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Stanford University, Stanford;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjun","family":"Shi","sequence":"additional","affiliation":[{"name":"Charles and Jane Pak Center of Mineral Metabolism, University of Texas Southwestern Medical Center, Dallas, Texas; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Funder","sequence":"additional","affiliation":[{"name":"Prince Henrys Institute, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orson W.","family":"Moe","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine,"},{"name":"Charles and Jane Pak Center of Mineral Metabolism, University of Texas Southwestern Medical Center, Dallas, Texas; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Pearce","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, and"},{"name":"Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.1.79"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.86.5.613"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.042903"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2913"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.404"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00308.x"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3971"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.16.9424"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00965.x"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.4.8095"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-09-03129.2000"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.03629.x"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2514"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1989.256.4.F672"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000600200"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1994.266.5.F767"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008121255"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21399"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.4.2035"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-002-0982-y"},{"key":"B21a","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00579.2007"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-002-0993-8"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/361549a0"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00240.2006"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00229.2004"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00484.2005"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10430"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200420083"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000013702.73570.3B"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011338200"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000120368.59693.A8"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/ng0396-325"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.21.11710"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.276.3.G567"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1990.315"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00044.x"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.146191"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00365.2001"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000098700.70804.D3"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1042\/bj3390319"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1042\/bj3440189"},{"key":"B42","doi-asserted-by":"crossref","first-page":"12032","DOI":"10.1016\/S0021-9258(17)32677-7","volume":"269","author":"Lee WS","year":"1994","journal-title":"J Biol Chem"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00241-0"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.4.F675"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-01-0027"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1727"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115487"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.24.16973"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2003.10.043"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00027.2007"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.11.3024"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1732"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.64.082101.143243"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00068.2002"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-002-0823-z"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.079624"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.2.F303"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00480.x"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215696"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107768200"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.7.3978"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00075.2010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T06:43:09Z","timestamp":1636612989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00075.2010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":62,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1152\/ajprenal.00075.2010"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00075.2010","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.5752969.5736078","asserted-by":"object"}]},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12]]}}}