{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:07:31Z","timestamp":1792649251175,"version":"4.5.0"},"reference-count":39,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01DK110622"],"award-info":[{"award-number":["R01DK110622"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01DK111861"],"award-info":[{"award-number":["R01DK111861"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["1S10RR019911-01"],"award-info":[{"award-number":["1S10RR019911-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001463","name":"American Society of Nephrology","doi-asserted-by":"publisher","award":["Gottschalk Research Scholar Grant"],"award-info":[{"award-number":["Gottschalk Research Scholar Grant"]}],"id":[{"id":"10.13039\/100001463","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:p>\n                    The development of chronic kidney disease (CKD) is associated with the loss of functional nephrons. However, there are no methods to directly measure nephron number in living subjects. Thus, there are no methods to track the early stages of progressive CKD before changes in total renal function. In this work, we used cationic ferritin-enhanced magnetic resonance imaging (CFE-MRI) to enable measurements of glomerular number ( N\n                    <jats:sub>glom<\/jats:sub>\n                    ) and apparent glomerular volume (aV\n                    <jats:sub>glom<\/jats:sub>\n                    ) in vivo in healthy wild-type (WT) mice ( n = 4) and mice with oligosyndactylism (Os\n                    <jats:sup>\/+<\/jats:sup>\n                    ; n = 4), a model of congenital renal hypoplasia leading to nephron reduction. We validated in vivo measurements of N\n                    <jats:sub>glom<\/jats:sub>\n                    and aV\n                    <jats:sub>glom<\/jats:sub>\n                    by high-resolution ex vivo MRI. CFE-MRI measured a mean N\n                    <jats:sub>glom<\/jats:sub>\n                    of 12,220\u2009\u00b1\u20092,028 and 6,848\u2009\u00b1\u20091,676 (means\u2009\u00b1\u2009SD) for WT and Os\n                    <jats:sup>\/+<\/jats:sup>\n                    mouse kidneys in vivo, respectively. N\n                    <jats:sub>glom<\/jats:sub>\n                    measured in all mice in vivo using CFE-MRI varied by an average 15% from N\n                    <jats:sub>glom<\/jats:sub>\n                    measured ex vivo in the same kidney (\u03b1\u2009=\u20090.05, P = 0.67). To confirm that CFE-MRI can also be used to track nephron endowment longitudinally, a WT mouse was imaged three times by CFE-MRI over 2 wk. Values of N\n                    <jats:sub>glom<\/jats:sub>\n                    measured in vivo in the same kidney varied within ~3%. Values of aV\n                    <jats:sub>glom<\/jats:sub>\n                    calculated from CFE-MRI in vivo were significantly different (~15% on average, P &lt; 0.01) from those measured ex vivo, warranting further investigation. This is the first report of direct measurements of N\n                    <jats:sub>glom<\/jats:sub>\n                    and aV\n                    <jats:sub>glom<\/jats:sub>\n                    in healthy and diseased mice in vivo.\n                  <\/jats:p>","DOI":"10.1152\/ajprenal.00078.2019","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T14:14:36Z","timestamp":1563977676000},"page":"F865-F873","source":"Crossref","is-referenced-by-count":35,"title":["In vivo measurements of kidney glomerular number and size in healthy and Os\n                    <sup>\/+<\/sup>\n                    mice using MRI"],"prefix":"10.1152","volume":"317","author":[{"given":"Edwin J.","family":"Baldelomar","sequence":"first","affiliation":[{"name":"Mallinckrodt Institute of Radiology, Washington University in St. Louis, St. Louis, Missouri"},{"name":"Department of Physics, University of Hawai\u2019i at M\u0101noa, Honolulu, Hawaii"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Charlton","sequence":"additional","affiliation":[{"name":"University of Virginia Children\u2019s Hospital, Department of Pediatrics, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly A.","family":"deRonde","sequence":"additional","affiliation":[{"name":"University of Virginia Children\u2019s Hospital, Department of Pediatrics, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin M.","family":"Bennett","sequence":"additional","affiliation":[{"name":"Mallinckrodt Institute of Radiology, Washington University in St. Louis, St. Louis, Missouri"},{"name":"Department of Biology, University of Hawai\u2019i at M\u0101noa, Honolulu, Hawaii"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00399.2017"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2015.316"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00092.2014"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.24301"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.24619"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00044.2011"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00714.2012"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21684"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110469"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/1.4.335"},{"key":"B11","volume-title":"National Chronic Kidney Disease Fact Sheet","author":"Centers for Disease Control and 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