{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:29:58Z","timestamp":1789694998767,"version":"4.0.1"},"reference-count":22,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:p>Chronic primary polydipsia (POLY) in humans is associated with impaired urinary concentrating ability. However, the molecular mechanisms responsible for this finding have not been elucidated. The purpose of this study was to examine the effect of chronic primary POLY on water metabolism and renal aquaporin (AQP) water channels and sodium and urea transporter abundance in rats. Primary POLY was induced in male Sprague-Dawley rats by daily administration of 15 g powdered rat chow mixed in 100 ml water for 10 days. Control rats (CTL) received 15 g powdered rat chow per day and ad libitum drinking water. Rats were studied following this period before further intervention and with a 36-h period of water deprivation to examine maximal urinary concentrating ability. At baseline, POLY rats demonstrated significantly greater water intake (100 \u00b1 1 vs. 22 \u00b1 2 ml\/day, P &lt; 0.0001) and urinary output (80 \u00b1 1 vs. 11 \u00b1 1 ml\/day, P &lt; 0.0001) and decreased urinary osmolality (159 \u00b1 13 vs. 1,365 \u00b1 188 mosmol\/kgH<jats:sub>2<\/jats:sub>O, P &lt; 0.001) compared with CTL rats. These findings were accompanied by decreased inner medulla AQP-2 protein abundance in POLY rats compared with CTL rats before water deprivation (76 \u00b1 2 vs. 100 \u00b1 7% CTL mean, P &lt; 0.007). With water deprivation, maximal urinary osmolality was impaired in POLY vs. CTL rats (2,404 \u00b1 148 vs. 3,286 \u00b1 175 mosmol\/kgH<jats:sub>2<\/jats:sub>O, P &lt; 0.0005). This defect occurred despite higher plasma vasopressin concentrations and similar medullary osmolalities in POLY rats. In response to 36-h water deprivation, inner medulla AQP-2 protein abundance was decreased in POLY rats compared with CTL rats (65 \u00b1 5 vs. 100 \u00b1 5% CTL mean, P &lt; 0.0006). No significant differences were noted in renal protein abundance of either AQP-3 or AQP-4 or sodium and urea transporters. We conclude that the impaired urinary concentrating ability associated with primary POLY in rats is due to impaired osmotic equilibration in the collecting duct that is mediated primarily by decreased AQP-2 protein abundance.<\/jats:p>","DOI":"10.1152\/ajprenal.00085.2003","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:39:33Z","timestamp":1425415173000},"page":"F965-F971","source":"Crossref","is-referenced-by-count":44,"title":["Effect of primary polydipsia on aquaporin and sodium transporter abundance"],"prefix":"10.1152","volume":"285","author":[{"given":"Melissa A.","family":"Cadnapaphornchai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra N.","family":"Summer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandor","family":"Falk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua M.","family":"Thurman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Knepper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Schrier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Apostol E, Ecelbarger CA, Terris J, Bradford AD, Andrews P, and Knepper M.Reduced renal medullary water channel expression in puromycin aminonucleoside-induced nephrotic syndrome.J Am Soc Nephrol8: 15-24, 1997.","DOI":"10.1681\/ASN.V8115"},{"key":"REF2","unstructured":"Berl Tand Schrier RW.Disorders of water metabolism. In:Renal and Electrolyte Disorders, edited by Schrier RW. Philadelphia, PA: Lippincott Williams & Wilkins, 2002."},{"key":"REF3","doi-asserted-by":"crossref","unstructured":"Bradford AD, Terris JM, Ecelbarger CA, Klein JD, Sands JM, Chou CL, and Knepper MA.97- And 117-kDa forms of collecting duct urea transporter UT-A1 are due to different states of glycosylation.Am J Physiol Renal Physiol281: F133-F143, 2001.","DOI":"10.1152\/ajprenal.2001.281.1.F133"},{"key":"REF4","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1957.sp005873"},{"key":"REF5","unstructured":"De Wardener HEand Nielsen S.Milestones in nephrology: the effect of a high water intake on the kidney's ability to concentrate the urine in man.J Am Soc Nephrol11: 980-987, 2000."},{"key":"REF6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1995.269.5.F663"},{"key":"REF7","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00984.x"},{"key":"REF8","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1988.255.5.F995"},{"key":"REF9","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.ndt.a027350"},{"key":"REF10","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197005212822107"},{"key":"REF11","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840170124"},{"key":"REF12","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200107193450304"},{"key":"REF13","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19590420303"},{"key":"REF14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.8.4296"},{"key":"REF15","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117863"},{"key":"REF16","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118628"},{"key":"REF17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.3.C655"},{"key":"REF18","doi-asserted-by":"crossref","unstructured":"Mudge GH, Foulkes J, and Gilman A.Effect of urea diuresis on renal excretion of electrolytes.Am J Physiol158: 218-230, 1949.","DOI":"10.1152\/ajplegacy.1949.158.2.218"},{"key":"REF19","unstructured":"Nielsen S, Knepper MA, Kwon TH, and Frokiaer J.Regulation of water balance: urine concentration and dilution. In:Diseases of the Kidney and Urinary Tract, edited by Schrier RW. Philadelphia, PA: Lippincott Williams & Wilkins, 2001."},{"key":"REF20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118736"},{"key":"REF21","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1995.269.6.F775"},{"key":"REF22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119312"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00085.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T09:38:09Z","timestamp":1651397889000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00085.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,11]]}},"alternative-id":["10.1152\/ajprenal.00085.2003"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00085.2003","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11]]}}}