{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:15:11Z","timestamp":1792653311482,"version":"4.5.0"},"reference-count":47,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p>Several members of the Na-HCO[Formula: see text] cotransporter (NBC) family have recently been identified functionally and partly characterized, including rkNBC1, NBCn1, and NBC3. Regulation of these NBCs may play a role in the maintenance of intracellular pH and in the regulation of renal acid-base balance. However, it is unknown whether the expressions of these NBCs are regulated in response to changes in acid-base status. We therefore tested whether chronic metabolic acidosis (CMA) affects the abundance of these NBCs in kidneys using two conventional protocols. In protocol 1, rats were treated with NH<jats:sub>4<\/jats:sub>Cl in their drinking water (12 \u00b1 1 mmol \u00b7 rat<jats:sup>\u22121<\/jats:sup>\u00b7 day<jats:sup>\u22121<\/jats:sup>) for 2 wk with free access to water ( n = 8). Semiquantitative immunoblotting demonstrated that whole kidney abundance of NBCn1 and NBC3 in rats with CMA was dramatically increased to 995 \u00b1 87 and 224 \u00b1 35%, respectively, of control levels ( P &lt; 0.05), whereas whole kidney rkNBC1 was unchanged (88 \u00b1 14%). In protocol 2, rats were given NH<jats:sub>4<\/jats:sub>Cl in their food (10 \u00b1 1 mmol \u00b7 rat<jats:sup>\u22121<\/jats:sup>\u00b7 day<jats:sup>\u22121<\/jats:sup>) for 7 days, with a fixed daily water intake ( n = 6). Consistent with protocol 1, whole kidney abundances of NBCn1 (262 \u00b1 42%) and NBC3 (160 \u00b1 31%) were significantly increased compared with controls ( n = 6), whereas whole kidney rkNBC1 was unchanged (84 \u00b1 17%). In both protocols, immunocytochemistry confirmed upregulation of NBCn1 and NBC3 with no change in the segmental distribution along the nephron. Consistent with the increase in NBCn1, measurements of pH transients in medullary thick ascending limb (mTAL) cells in kidney slices revealed two- to threefold increases in DIDS- sensitive, Na<jats:sup>+<\/jats:sup>-dependent HCO[Formula: see text] uptake in rats with CMA. In conclusion, CMA is associated with a marked increase in the abundance of NBCn1 in the mTAL and NBC3 in intercalated cells, whereas the abundance of NBC1 in the proximal tubule was not altered. The increased abundance of NBCn1 may play a role in the reabsorption of NH[Formula: see text] in the mTAL and increased NBC3 in reabsorbing HCO[Formula: see text].<\/jats:p>","DOI":"10.1152\/ajprenal.00104.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:33:46Z","timestamp":1425414826000},"page":"F341-F351","source":"Crossref","is-referenced-by-count":96,"title":["Chronic metabolic acidosis upregulates rat kidney Na-HCO 3 \u2212 cotransporters NBCn1 and NBC3 but not NBC1"],"prefix":"10.1152","volume":"282","author":[{"given":"Tae-Hwan","family":"Kwon","sequence":"first","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark;"},{"name":"Department of Physiology, School of Medicine, Dongguk University, Kyungju 780-714, Korea; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiaan","family":"Fulton","sequence":"additional","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Wang","sequence":"additional","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira","family":"Kurtz","sequence":"additional","affiliation":[{"name":"Division of Nephrology, University of California Los Angeles School of Medicine, Los Angeles, CA 90095-1689"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen","family":"Fr\u00f8ki\u00e6r","sequence":"additional","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Aalkj\u00e6r","sequence":"additional","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren","family":"Nielsen","sequence":"additional","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018539"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.274.3.F628"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113074"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.1.79"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.271.4.F917"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.6.F903"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115268"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.81.1.53"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1989.257.2.F170"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.274.6.F1119"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/35014615"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1982.242.5.F499"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019169"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.271.3.F619"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1988.255.1.F57"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1990.258.5.F1345"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1985.248.4.F459"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900102"},{"key":"B19","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1681\/ASN.V105935","volume":"10","author":"Kim GH","year":"1999","journal-title":"J Am Soc Nephrol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.279.3.F459"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869723"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1985.249.1.F62"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.278.2.F327"},{"key":"B24","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1152\/ajplegacy.1967.212.6.1327","volume":"212","author":"Landwehr DM","year":"1967","journal-title":"Am J Physiol"},{"key":"B25","first-page":"268","volume":"51","author":"Madsen KM","year":"1984","journal-title":"Lab Invest"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117863"},{"key":"B27","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1681\/ASN.V11122179","volume":"11","author":"Maunsbach AB","year":"2000","journal-title":"J Am Soc Nephrol"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108830"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1997.273.3.F340"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113750"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.279.6.F1014"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.23.16569"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.277.6.F974"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.699"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.274.2.F425"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.1.F27"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112948"},{"key":"B38","first-page":"419","volume":"43","author":"Soleimani M","year":"1995","journal-title":"J Invest Med"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110986"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.271.2.F414"},{"key":"B41","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1681\/ASN.V481546","volume":"4","author":"Verlander JW","year":"1994","journal-title":"J Am Soc Nephrol"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.279.5.F901"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1989.256.4.F549"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1990.258.1.F75"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.4.F606"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.10.5808"},{"key":"B47","first-page":"12A","volume":"11","author":"Yip KP","year":"2000","journal-title":"J Am Soc Nephrol"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00104.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T09:33:50Z","timestamp":1651397630000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00104.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2,1]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,2,1]]}},"alternative-id":["10.1152\/ajprenal.00104.2001"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00104.2001","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2,1]]}}}