{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T17:48:21Z","timestamp":1784224101361,"version":"3.55.0"},"reference-count":36,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2013,9,1]]},"abstract":"<jats:p>In the present study, the role of 5-HT<jats:sub>3<\/jats:sub>receptors in pudendal neuromodulation of bladder activity and its interaction with opioid receptors were investigated in anesthetized cats. The bladder was distended with either saline to induce normal bladder activity or with 0.25% acetic acid (AA) to induce bladder overactivity. Pudendal afferent nerves were activated by 5-Hz stimulation at multiples of the threshold (T) intensity for the induction of anal twitching. AA irritation significantly reduced bladder capacity to 16.5 \u00b1 3.3% of saline control capacity, whereas pudendal nerve stimulation (PNS) at 1.5\u20132 and 3\u20134 T restored the capacity to 82.0 \u00b1 12% ( P = 0.0001) and 98.6 \u00b1 15% ( P &lt; 0.0001), respectively. Cumulative doses (1\u20133 mg\/kg iv) of ondansetron, a 5-HT<jats:sub>3<\/jats:sub>receptor antagonist, eliminated low-intensity (1.5\u20132 T) PNS inhibition and reduced high-intensity (3\u20134 T) PNS inhibition of bladder overactivity. During saline distention, PNS at 1.5\u20132 and 3\u20134 T significantly increased bladder capacity to 173.2 \u00b1 26.4% ( P = 0.036) and 193.2 \u00b1 22.5% ( P = 0.008), respectively, of saline control capacity, but ondansetron (0.003\u20133 mg\/kg iv) did not alter PNS inhibition. Ondansetron (0.1\u20133 mg\/kg) also significantly ( P &lt; 0.05) increased control bladder capacity (50\u2013200%) during either AA irritation or saline distention. In both conditions, the effects of low- and high-intensity PNS were not significantly different. After ondansetron (3 mg\/kg) treatment, naloxone (1 mg\/kg iv) significantly ( P &lt; 0.05) decreased control bladder capacity (40\u201370%) during either AA irritation or saline distention but failed to affect PNS inhibition. This study revealed that activation of 5-HT<jats:sub>3<\/jats:sub>receptors has a role in PNS inhibition of bladder overactivity. It also indicated that 5-HT<jats:sub>3<\/jats:sub>receptor antagonists might be useful for the treatment of overactive bladder symptoms.<\/jats:p>","DOI":"10.1152\/ajprenal.00105.2013","type":"journal-article","created":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T08:28:15Z","timestamp":1372926495000},"page":"F663-F671","source":"Crossref","is-referenced-by-count":26,"title":["Involvement of 5-HT<sub>3<\/sub>receptors in pudendal inhibition of bladder overactivity in cats"],"prefix":"10.1152","volume":"305","author":[{"given":"Zeyad","family":"Schwen","sequence":"first","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Matsuta","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; 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