{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:03:36Z","timestamp":1791263016555,"version":"4.1.0"},"reference-count":53,"publisher":"American Physiological Society","issue":"6","funder":[{"name":"The American Heart Association, Post-doc fellowship","award":["0825465E"],"award-info":[{"award-number":["0825465E"]}]},{"name":"The America Heart Association, National Scientist Development Grant","award":["10SDG3770010"],"award-info":[{"award-number":["10SDG3770010"]}]},{"name":"National Institute of Health","award":["DK044628"],"award-info":[{"award-number":["DK044628"]}]},{"name":"National Institute of Health","award":["HL074167"],"award-info":[{"award-number":["HL074167"]}]},{"name":"National Institute of Health","award":["HL095499"],"award-info":[{"award-number":["HL095499"]}]},{"name":"National Institute of Health","award":["HL69999"],"award-info":[{"award-number":["HL69999"]}]},{"name":"National Institute of Health","award":["HL098135"],"award-info":[{"award-number":["HL098135"]}]},{"name":"National Institute of Health","award":["HL095499"],"award-info":[{"award-number":["HL095499"]}]},{"name":"National Institute of Health","award":["HL69999"],"award-info":[{"award-number":["HL69999"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2016,3,15]]},"abstract":"<jats:p>Inflammation contributes to ANG II-associated impairment of renal autoregulation and microvascular P2X<jats:sub>1<\/jats:sub>receptor signaling, but its role in renal autoregulation in mineralocorticoid-induced hypertension is unknown. Autoregulatory behavior was assessed using the blood-perfused juxtamedullary nephron preparation. Hypertension was induced in uninephrectomized control rats (UNx) by subcutaneous implantation of a DOCA pellet plus administration of 1% NaCl in the drinking water (DOCA-salt) for 3 wk. DOCA-salt rats developed hypertension that was unaltered by anti-inflammatory treatment with pentosan polysulfate (DOCA-salt+PPS) but was suppressed with \u201ctriple therapy\u201d (hydrochlorothiazide, hydralazine, and reserpine; DOCA-salt+TTx). Baseline arteriolar diameters were similar across all groups. UNx rats exhibited pressure-dependent vasoconstriction with diameters declining to 69 \u00b1 2% of control at 170 mmHg, indicating intact autoregulation. DOCA-salt treatment significantly blunted this pressure-mediated vasoconstriction. Diameters remained between 91 \u00b1 4 and 98 \u00b1 3% of control over 65\u2013170 mmHg, indicating impaired autoregulation. In contrast, pressure-mediated vasoconstriction was preserved in DOCA-salt+PPS and DOCA-salt+TTx rats, reaching 77 \u00b1 7 and 75 \u00b1 3% of control at 170 mmHg, respectively. ATP is required for autoregulation via P2X<jats:sub>1<\/jats:sub>receptor activation. ATP- and \u03b2,\u03b3-methylene ATP (P2X<jats:sub>1<\/jats:sub>receptor agonist)-mediated vasoconstriction were markedly attenuated in DOCA-salt rats compared with UNx ( P &lt; 0.05), but significantly improved by PPS or TTx ( P &lt; 0.05 vs. DOCA-salt) treatment. Arteriolar responses to adenosine and UTP (P2Y<jats:sub>2<\/jats:sub>receptor agonist) were unaffected by DOCA-salt treatment. PPS and TTx significantly reduced MCP-1 and protein excretion in DOCA-salt rats. These results support the hypothesis that hypertension triggers inflammatory cascades but anti-inflammatory treatment preserves renal autoregulation in DOCA-salt rats, most likely by normalizing renal microvascular reactivity to P2X<jats:sub>1<\/jats:sub>receptor activation.<\/jats:p>","DOI":"10.1152\/ajprenal.00110.2015","type":"journal-article","created":{"date-parts":[[2015,12,24]],"date-time":"2015-12-24T04:17:00Z","timestamp":1450930620000},"page":"F456-F465","source":"Crossref","is-referenced-by-count":8,"title":["Pentosan polysulfate preserves renal microvascular P2X<sub>1<\/sub>receptor reactivity and autoregulatory behavior in DOCA-salt hypertensive rats"],"prefix":"10.1152","volume":"310","author":[{"given":"Zhengrong","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Physiology, Georgia Regents University, Augusta, Georgia;"},{"name":"Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean T.","family":"Singletary","sequence":"additional","affiliation":[{"name":"Department of Physiology, Georgia Regents University, Augusta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haword","family":"Cha","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama; and"},{"name":"School of Natural Sciences, University of California, Merced, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin P.","family":"Van Beusecum","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony K.","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Physiology, Georgia Regents University, Augusta, Georgia;"},{"name":"Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer S.","family":"Pollock","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Pollock","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward W.","family":"Inscho","sequence":"additional","affiliation":[{"name":"Department of Physiology, Georgia Regents University, Augusta, Georgia;"},{"name":"Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e32835b36c1"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00929.x"},{"key":"B3","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1681\/ASN.V12102080","volume":"12","author":"Bobadilla NA","year":"2001","journal-title":"J Am Soc Nephrol"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2010.05428.x"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2006.01610.x"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00466.2007"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.098806"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12471"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00367.2010"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(96)00010-4"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvu249"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00281.2007"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.2174\/15701611113116660152"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00743.2009"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00286.2012"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/BF00382688"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00714.x"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.110.163576"},{"key":"B19","first-page":"608","volume":"121","author":"Imig JD","year":"1993","journal-title":"J Lab Clin Med"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.108.119982"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.110.168955"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI18499"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.2004.01317.x"},{"key":"B24","first-page":"S178","volume":"10","author":"Inscho EW","year":"1999","journal-title":"J Am Soc Nephrol"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1042\/cs1030131"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.35.3.400"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfn301"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.099499"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/694312"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1159\/000051064"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.098798"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.154"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00098.2009"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12412"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.33.1.389"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1979.237.1.F63"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00071.2002"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.27.791"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00016.2010"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9629(15)33943-4"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)43243-5"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/hr.2010.148"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012010017"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1002\/path.3964"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2013.197"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1042\/CS20130407"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.65.050102.085738"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504256200"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00012.2009"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1177\/1535370214565970"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.9.060906.151959"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.93"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000179584.39937.41"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00110.2015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T17:36:58Z","timestamp":1653932218000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00110.2015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,15]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,3,15]]}},"alternative-id":["10.1152\/ajprenal.00110.2015"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00110.2015","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,15]]}}}