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This study tested whether deletion of PKC-\u03b5 decreases dysfunction of renal cortical mitochondria and improves kidney function after renal ischemia. PKC-\u03b5 levels in mitochondria of ischemic kidneys increased 24 h after ischemia. Complex I- and complex II-coupled state 3 respirations were reduced 44 and 27%, respectively, in wild-type (WT) but unchanged and increased in PKC-\u03b5-deficient (KO) mice after ischemia. Respiratory control ratio coupled to glutamate\/malate oxidation decreased 50% in WT but not in KO mice. Activities of complexes I, III, and IV were decreased 59, 89, and 61%, respectively, in WT but not in KO ischemic kidneys. Proteomics revealed increases in levels of ATP synthase (\u03b1-subunit), complexes I and III, cytochrome oxidase, \u03b1-ketoglutarate dehydrogenase, and thioredoxin-dependent peroxide reductase after ischemia in KO but not in WT animals. PKC-\u03b5 deletion prevented ischemia-induced increases in oxidant production. Plasma creatinine levels increased 12-fold in WT and 3-fold in KO ischemic mice. PKC-\u03b5 deletion reduced tubular necrosis, brush border loss, and distal segment damage in ischemic kidneys. PKC-\u03b5 activation in hypoxic RPTC in primary culture exacerbated, whereas PKC-\u03b5 inhibition reduced, decreases in: 1) complex I- and complex II-coupled state 3 respirations and 2) activities of complexes I, III, and IV. We conclude that PKC-\u03b5 activation mediates 1) dysfunction of complexes I and III of the respiratory chain, 2) oxidant production, 3) morphological damage to the kidney, and 4) decreases in renal functions after ischemia.<\/jats:p>","DOI":"10.1152\/ajprenal.00115.2016","type":"journal-article","created":{"date-parts":[[2016,10,20]],"date-time":"2016-10-20T01:43:46Z","timestamp":1476927826000},"page":"F109-F120","source":"Crossref","is-referenced-by-count":17,"title":["Deletion of protein kinase C-\u03b5 attenuates mitochondrial dysfunction and ameliorates ischemic renal injury"],"prefix":"10.1152","volume":"312","author":[{"given":"Grazyna","family":"Nowak","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock, Arkansas; 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