{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T13:48:05Z","timestamp":1773150485516,"version":"3.50.1"},"reference-count":35,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p>The effect of arginine vasopressin (AVP) and\/or atrial natriuretic peptide (ANP) on the regulation of intracellular pH (pH<jats:sub>i<\/jats:sub>) via H<jats:sup>+<\/jats:sup>-ATPase and of cytosolic calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) was investigated in Madin-Darby canine kidney (MDCK) cells by the fluorescent probes BCECF-AM and fluo-4-AM, respectively. The pH<jats:sub>i<\/jats:sub>recovery rate was examined after intracellular acidification following an NH<jats:sub>4<\/jats:sub>Cl pulse, in the presence of zero Na<jats:sup>+<\/jats:sup>plus Schering 28080 (a specific inhibitor of H<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase). AVP (10<jats:sup>-12<\/jats:sup>-10<jats:sup>-6<\/jats:sup>M) increased the rate of pH<jats:sub>i<\/jats:sub>recovery and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in a dose-dependent manner. V<jats:sub>1<\/jats:sub>- or V<jats:sub>2<\/jats:sub>-receptor antagonists impaired the effect of AVP on both processes, and DDAVP (10<jats:sup>-12<\/jats:sup>-10<jats:sup>-6<\/jats:sup>M; a V<jats:sub>2<\/jats:sub>-selective agonist) caused a dose-dependent stimulation of them. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>or cAMP (as increased by 10<jats:sup>-5<\/jats:sup>M thapsigargin or 8-BrcAMP, respectively) alone had no effect on H<jats:sup>+<\/jats:sup>-ATPase, but their synergic action was necessary to stimulate H<jats:sup>+<\/jats:sup>-ATPase. In agreement with these findings, ANP (10<jats:sup>-6<\/jats:sup>M) or dimethyl-BAPTA-AM (5 \u00d7 10<jats:sup>-5<\/jats:sup>M), impairing the increase of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in response to AVP, blocks the stimulatory effect of AVP on H<jats:sup>+<\/jats:sup>-ATPase.<\/jats:p>","DOI":"10.1152\/ajprenal.00121.2003","type":"journal-article","created":{"date-parts":[[2004,1,31]],"date-time":"2004-01-31T01:13:21Z","timestamp":1075511601000},"page":"F402-F408","source":"Crossref","is-referenced-by-count":16,"title":["Arginine vasopressin stimulates H<sup>+<\/sup>-ATPase in MDCK cells via V<sub>1<\/sub>(cell Ca<sup>2+<\/sup>) and V<sub>2<\/sub>(cAMP) receptors"],"prefix":"10.1152","volume":"286","author":[{"given":"Maria","family":"Oliveira-Souza","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Instituto de Ci\u00eancias Biom\u00e9dicas, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-900, 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