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Renal blood flow was quickly restored, whereas the glomerular filtration rate remained halved 30 min after IRI. Tubular cell activity was intact as judged from the unaffected Cr-EDTA U\/P concentration ratio. In vivo, the fractional clearance (\u03b8) for albumin increased 16 times. In rats subjected to cIPK starting 30 min after in vivo IRI, \u03b8<jats:sub>albumin<\/jats:sub>was 15 times and \u03b8<jats:sub>Ficoll_36\u00c5<\/jats:sub>1.8 times higher than in control cIPKs. According to the heterogeneous charged fiber model, IRI reduced the fiber charge density to 38% of control ( P &lt; 0.01, n = 7). Morphometric analysis with electron microscopy did not reveal any changes in the podocytes or the glomerular basement membrane (GBM) after IRI, suggesting more subtle changes of the GBM and\/or the endothelial glycocalyx. We conclude that mild renal IRI induces formation of reactive oxygen species, massive proteinuria, and loss of charged fibers with no apparent change in morphology. These novel findings stress the importance of other components of the barrier, such as proteoglycans produced by the glomerular cells, and provide a tentative explanation for the mechanisms behind proteinuria in glomerulonephritis, for example.<\/jats:p>","DOI":"10.1152\/ajprenal.00152.2006","type":"journal-article","created":{"date-parts":[[2007,3,21]],"date-time":"2007-03-21T23:57:48Z","timestamp":1174521468000},"page":"F1802-F1809","source":"Crossref","is-referenced-by-count":40,"title":["Mild renal ischemia-reperfusion reduces charge and size selectivity of the glomerular barrier"],"prefix":"10.1152","volume":"292","author":[{"given":"Maria","family":"Andersson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf","family":"Nilsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Hjalmarsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00f6rje","family":"Haraldsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny S\u00f6rensson","family":"Nystr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000074311.71224.20"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006867214448"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00202.2004"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1997.273.3.F430"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(71)90528-5"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1007\/BF00788496"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23577"},{"key":"R8","doi-asserted-by":"crossref","unstructured":"Deen WM, Lazzara MJ, Myers BD.Structural determinants of glomerular permeability.Am J Physiol Renal Physiol281: F579\u2013F596, 2001.","DOI":"10.1152\/ajprenal.2001.281.4.F579"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(88)90504-4"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Haraldsson B, S\u00f6rensson J.Why do we not all have proteinuria? 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