{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:12:07Z","timestamp":1774278727821,"version":"3.50.1"},"reference-count":31,"publisher":"American Physiological Society","issue":"6","funder":[{"DOI":"10.13039\/100006792","name":"Hartwell Foundation","doi-asserted-by":"publisher","award":["60036802LCH"],"award-info":[{"award-number":["60036802LCH"]}],"id":[{"id":"10.13039\/100006792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2019,12,1]]},"abstract":"<jats:p>We have defined a population of stem cell antigen (Sca)-1<jats:sup>+<\/jats:sup>\/CD34<jats:sup>+<\/jats:sup>\/lin<jats:sup>\u2212<\/jats:sup>mesenchymal stem cells in the mouse urinary bladder. These cells are reduced after partial bladder outlet obstruction (PO). To test the role of Sca-1 expressed by these cells, we analyzed bladders from Sca-1 knockout (KO) mice in both uninjured male mice and male mice subjected to PO. We found that loss of Sca-1 alone had little effect on bladder development or function but reduced the total number of mesenchymal stem cells by 30%. After PO, bladders from Sca-1-null KO male mice were larger, with more collagen and less muscle, than obstructed wild-type mice. Steady-state levels of caldesmon were significantly reduced and levels of fibroblast-specific protein 1 were significantly increased in Sca-1 KO mice compared with wild-type mice after PO. In investigating the effects of PO on cell proliferation, we found that loss of Sca-1 changed the timing of cell division in CD34<jats:sup>+<\/jats:sup>\/lin<jats:sup>\u2212<\/jats:sup>, collagen-producing, and smooth muscle cells. PO in combination with loss of Sca-1 drastically reduced the ability of CD34<jats:sup>+<\/jats:sup>\/lin<jats:sup>\u2212<\/jats:sup>cells to form colonies in vitro. Our findings therefore support the hypothesis that Sca-1 protects the bladder from fibrotic remodeling after obstruction, in part by influencing the proliferation of cells responding to the injury.<\/jats:p>","DOI":"10.1152\/ajprenal.00160.2019","type":"journal-article","created":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T13:16:21Z","timestamp":1568812581000},"page":"F1503-F1512","source":"Crossref","is-referenced-by-count":6,"title":["Stem cell antigen\/Ly6a protects against bladder fibrosis in mice"],"prefix":"10.1152","volume":"317","author":[{"given":"Nicholas M.","family":"Tassone","sequence":"first","affiliation":[{"name":"Pediatric Urology, Stanley Manne Children\u2019s Research Institute, Ann & Robert H. 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