{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T10:08:34Z","timestamp":1759226914248},"reference-count":56,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2002,7,1]]},"abstract":"<jats:p>Serum and glucocorticoid-regulated kinase-1 (SGK1) is a serine-threonine kinase that is regulated at the transcriptional level by numerous regulatory inputs, including mineralocorticoids, glucocorticoids, follicle-stimulating hormone, and osmotic stress. In the distal nephron, SGK1 is induced by aldosterone and regulates epithelial Na<jats:sup>+<\/jats:sup>channel-mediated transepithelial Na<jats:sup>+<\/jats:sup>transport. In other tissues, including liver and shark rectal gland, SGK1 is regulated by hypertonic stress and is thought to modulate epithelial Na<jats:sup>+<\/jats:sup>channel- and Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter-mediated Na<jats:sup>+<\/jats:sup>transport. In this report, we examined the regulation of SGK1 mRNA and protein expression and Na<jats:sup>+<\/jats:sup>currents in response to osmotic stress in A6 cells, a cultured cell line derived from Xenopus laevis distal nephron. We found that in contrast to hepatocytes and rectal gland cells, hypotonic conditions stimulated SGK1 expression and Na<jats:sup>+<\/jats:sup>transport in A6 cells. Moreover, a correlation was found between SGK1 induction and the later phase of activation of Na<jats:sup>+<\/jats:sup>transport in response to hypotonic treatment. When A6 cells were pretreated with an inhibitor of phosphatidylinositol 3-kinase (PI3K), Na<jats:sup>+<\/jats:sup>transport was blunted and only inactive forms of SGK1 were expressed. Surprisingly, these results demonstrate that both hypertonic and hypotonic stimuli can induce SGK1 gene expression in a cell type-dependent fashion. Moreover, these data lend support to the view that SGK1 contributes to the defense of extracellular fluid volume and tonicity in amphibia by mediating a component of the hypotonic induction of distal nephron Na<jats:sup>+<\/jats:sup>transport.<\/jats:p>","DOI":"10.1152\/ajprenal.00176.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:32:35Z","timestamp":1425414755000},"page":"F105-F113","source":"Crossref","is-referenced-by-count":50,"title":["Hypotonic induction of SGK1 and Na<sup>+<\/sup>transport in A6 cells"],"prefix":"10.1152","volume":"283","author":[{"given":"David J.","family":"Rozansky","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Pediatrics, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Departments of Medicine and of Cellular and Molecular Pharmacology, University of California, 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