{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:47:06Z","timestamp":1787017626847,"version":"build-2736575974"},"reference-count":23,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2019,5,1]]},"abstract":"<jats:p>\n                    Kidney normothermic machine perfusion (NMP) has historically used a 95% O\n                    <jats:sub>2<\/jats:sub>\n                    -5% CO\n                    <jats:sub>2<\/jats:sub>\n                    gas mixture. Using a porcine model of organ retrieval, NMP, and reperfusion, we tested the hypothesis that reducing perfusate oxygenation ([Formula: see text]) would be detrimental to renal function and cause injury. In the minimal ischemic injury experiment, kidneys sustained 10 min of warm ischemia and 2 h of static cold storage before 1 h of NMP with either 95%, 25%, or 12% O\n                    <jats:sub>2<\/jats:sub>\n                    with 5% CO\n                    <jats:sub>2<\/jats:sub>\n                    and N\n                    <jats:sub>2<\/jats:sub>\n                    balance. In the clinical injury experiment, kidneys with 10-min warm ischemia and 17-h static cold storage underwent 1-h NMP with the above gas combinations or 18-h static cold storage as a control. They were then reperfused with whole blood and 95% O\n                    <jats:sub>2<\/jats:sub>\n                    for 3 h. Overall, reducing [Formula: see text] did not significantly influence renal function in either experiment. Furthermore, there were no differences in the injury markers urinary neutrophil gelatinase-associated lipocalin or tissue high-motility group box protein 1. In the minimal ischemic injury experiment, a [Formula: see text] of 25% significantly reduced renal blood flow and increased vascular resistance. Oxygen delivery, consumption, and extraction (oxygen extraction ratio) were significantly greater at 95% [Formula: see text]. In the clinical injury experiment, renal blood flow was significantly increased at 25% [Formula: see text] and Na\n                    <jats:sup>+<\/jats:sup>\n                    excretion decreased. At 95% [Formula: see text], the oxygen content and oxygen extraction ratio were significantly increased. During reperfusion, renal blood flow was significantly increased in the 25% group. The control group pH was significantly decreased compared with the 25% group. Our data suggest that reducing [Formula: see text] during NMP does not have detrimental effects on renal function or markers of injury.\n                  <\/jats:p>","DOI":"10.1152\/ajprenal.00178.2018","type":"journal-article","created":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T10:55:35Z","timestamp":1550660135000},"page":"F823-F829","source":"Crossref","is-referenced-by-count":21,"title":["Physiological effects of altering oxygenation during kidney normothermic machine perfusion"],"prefix":"10.1152","volume":"316","author":[{"given":"Thomas D.","family":"Adams","sequence":"first","affiliation":[{"name":"Department of Surgery, University of Cambridge, Addenbrooke\u2019s Hospital, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A.","family":"Hosgood","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Cambridge, Addenbrooke\u2019s Hospital, Cambridge, United Kingdom"},{"name":"Department of Infection, Immunity and Inflammation, Transplant Group, University of Leicester, Leicester General Hospital, Leicester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Nicholson","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Cambridge, Addenbrooke\u2019s Hospital, Cambridge, United Kingdom"},{"name":"Department of Infection, Immunity and Inflammation, Transplant Group, University of Leicester, Leicester General Hospital, Leicester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1097\/TXD.0000000000000655"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000081044.37318.E3"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2004.11.043"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90230.2008"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00552.2010"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.12031"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.08.020"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.12034"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.9894"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001504"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2012.08.001"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0000000000000405"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001343"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(78)90046-4"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31826a44f6"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081386"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00710.2002"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.98.12030653"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.12036"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1002\/9781119975427.ch5"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200010270-00024"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.2001.tb00054.x"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/864946"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00178.2018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T13:22:11Z","timestamp":1567948931000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00178.2018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,1]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,5,1]]}},"alternative-id":["10.1152\/ajprenal.00178.2018"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00178.2018","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,1]]}}}