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We examined the changes in the abundance of the type 3 Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>exchanger (NHE3), Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase, the bumetanide-sensitive Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter (BSC-1), the thiazide-sensitive Na<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>cotransporter (TSC), and epithelial sodium channel (ENaC) subunits in kidneys of hypokalemic rats. Semiquantitative immunoblotting revealed that the abundance of BSC-1 (57%) and TSC (46%) were profoundly decreased in the inner stripe of the outer medulla (ISOM) and cortex\/outer stripe of the outer medulla (OSOM), respectively. These findings were confirmed by immunohistochemistry. Moreover, total kidney abundance of all ENaC subunits was significantly reduced in response to the hypokalemia: \u03b1-subunit (61%), \u03b2-subunit (41%), and \u03b3-subunit (60%), and this was confirmed by immunohistochemistry. In contrast, the renal abundance of NHE3 in hypokalemic rats was dramatically increased in cortex\/OSOM (736%) and ISOM (210%). Downregulation of BSC-1, TSC, and ENaC may contribute to the urinary concentrating defect, whereas upregulation of NHE3 may be compensatory to prevent urinary Na<jats:sup>+<\/jats:sup>loss and\/or to maintain intracellular pH levels.<\/jats:p>","DOI":"10.1152\/ajprenal.00186.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:36:32Z","timestamp":1425414992000},"page":"F1376-F1388","source":"Crossref","is-referenced-by-count":65,"title":["Altered expression of renal NHE3, TSC, BSC-1, and ENaC subunits in potassium-depleted rats"],"prefix":"10.1152","volume":"283","author":[{"given":"Marie-Louise","family":"Elkj\u00e6r","sequence":"first","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae-Hwan","family":"Kwon","sequence":"additional","affiliation":[{"name":"The Water and Salt Research Center, University of Aarhus, 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