{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:40:01Z","timestamp":1791322801800,"version":"4.3.1"},"reference-count":31,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2002,5,1]]},"abstract":"<jats:p>In the proximal tubule, the apical Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>exchanger identified as NHE3 mediates most NaCl and NaHCO<jats:sub>3<\/jats:sub>absorption. The purpose of this study was to analyze the long-term regulation of NHE3 during alkalosis induced by dietary NaHCO<jats:sub>3<\/jats:sub>loading and changes in NaCl intake. Sprague-Dawley rats exposed to a low-NaCl, high-NaCl, or NaHCO<jats:sub>3<\/jats:sub>diet for 6 days were studied. Renal cortical apical membrane vesicles (AMV) were prepared from treated and normal rats. Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>exchange was assayed as the initial rate of<jats:sup>22<\/jats:sup>Na<jats:sup>+<\/jats:sup>uptake in the presence of an outward H<jats:sup>+<\/jats:sup>gradient.<jats:sup>22<\/jats:sup>Na<jats:sup>+<\/jats:sup>uptake measured in the presence of high-dose 5-( N-ethyl- N-isopropyl) amiloride was not different among models. Changes in NaCl intake did not affect NHE3 activity, whereas NaHCO<jats:sub>3<\/jats:sub>loading inhibited<jats:sup>22<\/jats:sup>Na<jats:sup>+<\/jats:sup>uptake by 30%. AMV NHE3 protein abundance assessed by Western blot analysis was unaffected during changes in NaCl intake. During NaHCO<jats:sub>3<\/jats:sub>loading, NHE3 protein abundance was decreased by 65%. We conclude that proximal NHE3 adapts to chronic metabolic acid-base disorders but not to changes in dietary NaCl intake.<\/jats:p>","DOI":"10.1152\/ajprenal.00188.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:35:05Z","timestamp":1425414905000},"page":"F835-F843","source":"Crossref","is-referenced-by-count":29,"title":["Rat proximal NHE3 adapts to chronic acid-base disorders but not to chronic changes in dietary NaCl intake"],"prefix":"10.1152","volume":"282","author":[{"given":"Dominique","family":"Eladari","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Unit\u00e9 356, Institut F\u00e9d\u00e9ratif de Recherche 58, Universit\u00e9 Pierre et Marie Curie, 75270 Paris Cedex 06; and"},{"name":"H\u00f4pital Europ\u00e9en Georges Pompidou, Assistance Publique-H\u00f4pitaux de Paris, 75908 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Leviel","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Unit\u00e9 356, Institut F\u00e9d\u00e9ratif de Recherche 58, Universit\u00e9 Pierre et Marie Curie, 75270 Paris Cedex 06; 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