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Previous studies of the intrarenal distribution of V<jats:sub>2<\/jats:sub>Rs have been inconclusive with respect to segment- and cell-type-related V<jats:sub>2<\/jats:sub>R expression. Our study therefore aimed to present a high-resolution analysis of V<jats:sub>2<\/jats:sub>R mRNA expression in rat, mouse, and human kidney epithelia, supplemented with immunohistochemical data. Cell types of the renal tubule were identified histochemically using specific markers. Pronounced V<jats:sub>2<\/jats:sub>R signal in thick ascending limb (TAL) was corroborated functionally; phosphorylation of Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter type 2 (NKCC2) was established in cultured TAL cells from rabbit and in rats with diabetes insipidus that were treated with the V<jats:sub>2<\/jats:sub>R agonist desmopressin. We found solid expression of V<jats:sub>2<\/jats:sub>R mRNA in medullary TAL (MTAL), macula densa, connecting tubule, and cortical and medullary collecting duct and weaker expression in cortical TAL and distal convoluted tubule in all three species. Additional V<jats:sub>2<\/jats:sub>R immunostaining of kidneys and rabbit TAL cells confirmed our findings. In agreement with strong V<jats:sub>2<\/jats:sub>R expression in MTAL, kidneys from rats with diabetes insipidus and cultured TAL cells revealed sharp, selective increases in NKCC2 phosphorylation upon desmopressin treatment. Macula densa cells constitutively showed strong NKCC2 phosphorylation. Results suggest comparably significant effects of vasopressin-induced V<jats:sub>2<\/jats:sub>R signaling in MTAL and in connecting tubule\/collecting duct principal cells across the three species. 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