{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T22:43:35Z","timestamp":1726440215633},"reference-count":50,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:p> To investigate the responses of descending vasa recta (DVR) to deformation of the abluminal surface, we devised an automated method that controls duration and frequency of stimulation by utilizing a stream of buffer from a micropipette. During stimulation at one end of the vessel, fluorescent responses from fluo4 or bis[1,3-dibutylbarbituric acid-(5)] trimethineoxonol [DiBAC<jats:sub>4<\/jats:sub>(3)], indicating cytoplasmic calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>CYT<\/jats:sub>) or membrane potential, respectively, were recorded from distant cells. Alternately, membrane potential was recorded from DVR pericytes by nystatin whole cell patch-clamp. Mechanical stimulation elicited reversible [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>CYT<\/jats:sub> responses that increased with frequency. Individual pericyte responses along the vessel were initiated within a fraction of a second of one another. Those responses were inhibited by gap junction blockade with 18 \u03b2-glycyrrhetinic acid (100 \u03bcM) or phosphoinositide 3 kinase inhibition with 2-morpholin-4-yl-8-phenylchromen-4-one (50 \u03bcM). [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>CYT<\/jats:sub> responses were blocked by removal of extracellular Ca<jats:sup>2+<\/jats:sup> or L-type voltage-gated channel blockade with nifedipine (10 \u03bcM). At concentrations selective for the T-type channel blockade, mibefradil (100 nM) was ineffective. During mechanostimulation, pericytes rapidly depolarized, as documented with either DiBAC<jats:sub>4<\/jats:sub>(3) fluorescence or patch-clamp recording. Single stimuli yielded depolarizations of 22.5 \u00b1 2.2 mV while repetitive stimuli at 0.1 Hz depolarized pericytes by 44.2 \u00b1 4.0 mV. We conclude that DVR are mechanosensitive and that rapid transmission of signals along the vessel axis requires participation of gap junctions, L-type Ca<jats:sup>2+<\/jats:sup> channels, and pericyte depolarization. <\/jats:p>","DOI":"10.1152\/ajprenal.00220.2013","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T22:06:57Z","timestamp":1369260417000},"page":"F286-F294","source":"Crossref","is-referenced-by-count":8,"title":["Mural propagation of descending vasa recta responses to mechanical stimulation"],"prefix":"10.1152","volume":"305","author":[{"given":"Zhong","family":"Zhang","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristie","family":"Payne","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, University of Maryland School of 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