{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:24:10Z","timestamp":1782577450407,"version":"3.54.5"},"reference-count":49,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (National Council of Science and Technology, Mexico)","doi-asserted-by":"publisher","award":["222918"],"award-info":[{"award-number":["222918"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (National Council of Science and Technology, Mexico)","doi-asserted-by":"publisher","award":["155700"],"award-info":[{"award-number":["155700"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (National Council of Science and Technology, Mexico)","doi-asserted-by":"publisher","award":["261562"],"award-info":[{"award-number":["261562"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (National Council of Science and Technology, Mexico)","doi-asserted-by":"publisher","award":["235855"],"award-info":[{"award-number":["235855"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (National Council of Science and Technology, Mexico)","doi-asserted-by":"publisher","award":["261562"],"award-info":[{"award-number":["261562"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2018,2,1]]},"abstract":"<jats:p>Geranium seemannii Peyr is a perennial plant endemic to central Mexico that has been widely used for its diuretic effect, but the responsible compound of this effect is unknown as well as the mechanism by which the diuretic effect is achieved. Geraniin is one of the compounds isolated from this kind of geranium. This study was designed to determinate whether geraniin possesses diuretic activity and to elucidate the mechanism of action. Geraniin was extracted and purified from Geranium seemannii Peyr. Male Wistar rats were divided into four groups: 1) Control, 2) 75 mg\/kg of geraniin, 3) 20 mg\/kg of furosemide, and 4) 10 mg\/kg of hydrochlorothiazide. Each treatment was administered by gavage every 24 h for 7 days. The urinary excretion of electrolytes and the fractional excretion of sodium (FENa) were determined. To uncover the molecular target of geraniin, Xenopus laevis oocytes were microinjected with cRNAs encoding the Na<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>cotransporter (NCC) and the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter NKCC2 to functionally express these cotransporters. Geraniin significantly increased diuresis, natriuresis, and calciuresis to a similar extent as was observed in the furosemide-treated rats. Consistent with the furosemide-like effect, in X. laevis oocytes, geraniin significantly reduced the activity of NKCC2, with no effect on NCC activity. In contrast to furosemide, the effect of geraniin on NKCC2 was irreversible, apparently due to its inhibitory effect on heat shock protein 90. Our observations suggest that geraniin could have a potential role in the treatment of hypertension or edematous states.<\/jats:p>","DOI":"10.1152\/ajprenal.00221.2017","type":"journal-article","created":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T12:50:09Z","timestamp":1508331009000},"page":"F240-F250","source":"Crossref","is-referenced-by-count":10,"title":["Geraniin is a diuretic by inhibiting the Na<sup>+<\/sup>-K<sup>+<\/sup>-2Cl<sup>\u2212<\/sup>cotransporter NKCC2"],"prefix":"10.1152","volume":"314","author":[{"given":"Erika","family":"Moreno","sequence":"first","affiliation":[{"name":"Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Tlalpan, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Gayosso","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo Pachuca, Hidalgo, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R.","family":"Montejano","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo Pachuca, Hidalgo, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina","family":"Almaguer","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo Pachuca, Hidalgo, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Molecular Physiology Unit, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristino","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Tlalpan, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Mercado","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Tlalpan, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma A.","family":"Bobadilla","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Tlalpan, Mexico City, Mexico"},{"name":"Molecular Physiology Unit, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"Gamba","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Tlalpan, Mexico City, Mexico"},{"name":"Molecular Physiology Unit, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"},{"name":"Tecnol\u00f3gico de Monterrey, Escuela de Medicina y de Ciencias de la Salud, Monterrey, Nuevo Le\u00f3n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Sierra","sequence":"additional","affiliation":[{"name":"Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2782-1381","authenticated-orcid":false,"given":"Victoria","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Tlalpan, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","first-page":"1","volume":"12","author":"Barnes PM","year":"2008","journal-title":"Natl Health Stat Report"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1021\/jf030374m"},{"key":"B3","first-page":"A44","volume":"6","author":"Callahan LF","year":"2009","journal-title":"Prev Chronic Dis"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00412.2010"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<1118::AID-JSFA570>3.0.CO;2-9"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051360203"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1021\/jm00300a012"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki526"},{"key":"B10","doi-asserted-by":"crossref","first-page":"17713","DOI":"10.1016\/S0021-9258(17)32499-7","volume":"269","author":"Gamba G","year":"1994","journal-title":"J Biol Chem"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.139788"},{"key":"B12","first-page":"531","volume":"5","author":"Gayosso-De-Lucio JA","year":"2010","journal-title":"Nat Prod Commun"},{"key":"B13","first-page":"28","volume":"22","author":"Guo JS","year":"1987","journal-title":"Yao Xue Bao"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-006-0105-6"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.11.059"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2044.2009.06228.x"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1270749"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/ncpneuro0883"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1021\/np970171q"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.10.005"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200700381"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i25.7718"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00970.2011"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-013-1370-5"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-957838"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.279.1.F161"},{"key":"B29","first-page":"709","volume":"6","author":"Montejano-Rodr\u00edguez JR","year":"2013","journal-title":"J Pharm Res"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602614200"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.24.1145"},{"key":"B32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-7091-9363-1_1","volume":"66","author":"Okuda T","year":"1995","journal-title":"Fortschr Chem Org Naturst"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00382.2003"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00383.2003"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603773200"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00071.2005"},{"key":"B37","volume-title":"Lista de las Plantas \u00datiles del Estado de Hidalgo","author":"P\u00e9rez Escand\u00f3n BE","year":"2003"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1201\/b16504"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90278.2008"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1379\/CSC-22R.1"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1177\/1074248413497257"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.40.2937"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.02.006"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074266"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00284.2001"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-14-506"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90227.2008"},{"key":"B51","unstructured":"World Health Organization.Estrategia de la OMS Sobre la Medicina Tradicional 2002\u20132005(Online). WHO\/EDM\/TRM\/2002.1. www.who.int\/iris\/handle\/10665\/67163\/."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.07.023"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.01.102"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.3987\/R-1980-11-1743"},{"key":"B55","volume-title":"Estudio de la Relaci\u00f3n Planta-Hombre en los Municipios de Mineral del Monte, Mineral del Chico, Estado de Hidalgo","author":"Zamora Martinez LI","year":"1997"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00221.2017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T20:45:09Z","timestamp":1659645909000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00221.2017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,1]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2,1]]}},"alternative-id":["10.1152\/ajprenal.00221.2017"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00221.2017","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,1]]}}}