{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T07:14:49Z","timestamp":1775286889931,"version":"3.50.1"},"reference-count":65,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)","doi-asserted-by":"publisher","award":["RO1 DK071014"],"award-info":[{"award-number":["RO1 DK071014"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)","doi-asserted-by":"publisher","award":["RO1 DK92474"],"award-info":[{"award-number":["RO1 DK92474"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)","doi-asserted-by":"publisher","award":["F30 DK108456"],"award-info":[{"award-number":["F30 DK108456"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:p>\n                    Special high-K diets have cardioprotective effects and are often warranted in conjunction with diuretics such as furosemide for treating hypertension. However, it is not understood how a high-K diet (HK) influences the actions of diuretics on renal K\n                    <jats:sup>+<\/jats:sup>\n                    handling. Furosemide acidifies the urine by increasing acid secretion via the Na\n                    <jats:sup>+<\/jats:sup>\n                    -H\n                    <jats:sup>+<\/jats:sup>\n                    exchanger 3 (NHE3) in TAL and vacuolar H\n                    <jats:sup>+<\/jats:sup>\n                    -ATPase (V-ATPase) in the distal nephron. We previously found that an alkaline urine is required for large conductance Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -activated K\n                    <jats:sup>+<\/jats:sup>\n                    (BK)-\u03b1\u03b24-mediated K\n                    <jats:sup>+<\/jats:sup>\n                    secretion in mice on HK. We therefore hypothesized that furosemide could reduce BK-\u03b1\u03b24-mediated K\n                    <jats:sup>+<\/jats:sup>\n                    secretion by acidifying the urine. Treating with furosemide (drinking water) for 11 days led to decreased urine pH in both wild-type (WT) and BK-\u03b24-knockout mice (BK-\u03b24-KO) with increased V-ATPase expression and elevated plasma aldosterone levels. However, furosemide decreased renal K\n                    <jats:sup>+<\/jats:sup>\n                    clearance and elevated plasma [K\n                    <jats:sup>+<\/jats:sup>\n                    ] in WT but not BK-\u03b24-KO. Western blotting and immunofluorescence staining showed that furosemide treatment decreased cortical expression of BK-\u03b24 and reduced apical localization of BK-\u03b1 in connecting tubules. Addition of the carbonic anhydrase inhibitor, acetazolamide, to furosemide water restored urine pH along with renal K\n                    <jats:sup>+<\/jats:sup>\n                    clearance and plasma [K\n                    <jats:sup>+<\/jats:sup>\n                    ] to control levels. Acetazolamide plus furosemide also restored the cortical expression of BK-\u03b24 and BK-\u03b1 in connecting tubules. These results indicate that in mice adapted to HK, furosemide reduces BK-\u03b1\u03b24-mediated K\n                    <jats:sup>+<\/jats:sup>\n                    secretion by acidifying the urine.\n                  <\/jats:p>","DOI":"10.1152\/ajprenal.00223.2018","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T06:32:16Z","timestamp":1543386736000},"page":"F341-F350","source":"Crossref","is-referenced-by-count":8,"title":["Furosemide reduces BK-\u03b1\u03b24-mediated K\n                    <sup>+<\/sup>\n                    secretion in mice on an alkaline high-K\n                    <sup>+<\/sup>\n                    diet"],"prefix":"10.1152","volume":"316","author":[{"given":"Bangchen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, 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