{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:50:23Z","timestamp":1772164223935,"version":"3.50.1"},"reference-count":37,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>\n                    Salt reabsorption by the loop of Henle controls NaCl handling and blood pressure regulation. Increased oxidative stress stimulates NaCl transport in one specific segment of the loop of Henle called the thick ascending limb (TAL). The isoprostane 8-iso-prostaglandin-F2\u03b1 (8-iso-PGF2\u03b1) is one of the most abundant nonenzymatic lipid oxidation products and has been implicated in the development of hypertension. However, it is not known whether 8-iso-PGF2\u03b1 regulates transport or the mechanisms involved. Because protein kinase A (PKA) stimulates NaCl transport in several nephron segments, we hypothesized that 8-iso-PGF2\u03b1 increases NaCl transport in the cortical TAL (cTAL) via a PKA-dependent mechanism. We examined the effect of luminal 8-iso-PGF2\u03b1 on NaCl transport by measuring chloride absorption ( J\n                    <jats:sub>Cl<\/jats:sub>\n                    ) in isolated microperfused cTALs. Adding 8-iso-PGF2\u03b1 to the lumen increased J\n                    <jats:sub>Cl<\/jats:sub>\n                    by 54% (from 288.7 \u00b1 30.6 to 446.5 \u00b1 44.3 pmol\u00b7min\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mm\n                    <jats:sup>\u22121<\/jats:sup>\n                    ; P &lt; 0.01), while adding it to the bath enhanced J\n                    <jats:sub>Cl<\/jats:sub>\n                    by 35% (from 236.3 \u00b1 35.3 to 319.2 \u00b1 39.8 pmol\u00b7min\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mm\n                    <jats:sup>\u22121<\/jats:sup>\n                    ; P &lt; 0.05). This stimulation was blocked by Na-K-2Cl cotransporter inhibition. Next, we tested the role of cAMP. Basal cAMP in the cTAL was 18.6 \u00b1 1.6 fmol\u00b7min\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mm\n                    <jats:sup>\u22121<\/jats:sup>\n                    , and 8-iso-PGF2\u03b1 raised it to 35.1 \u00b1 1.4 fmol\u00b7min\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mm\n                    <jats:sup>\u22121<\/jats:sup>\n                    , an increase of 94% ( P &lt; 0.01). Because cAMP stimulates PKA, we measured J\n                    <jats:sub>Cl<\/jats:sub>\n                    using the PKA-selective inhibitor H89. In the presence of H89 (10 \u03bcM), 8-iso-PGF2\u03b1 failed to increase transport regardless of whether it was added to the lumen (216.1 \u00b1 16.7 vs. 209.7 \u00b1 23.8 pmol\u00b7min\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mm\n                    <jats:sup>\u22121<\/jats:sup>\n                    ; NS) or the bath (150.4 \u00b1 32.9 vs. 127.1 \u00b1 28.6 pmol\u00b7min\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mm\n                    <jats:sup>\u22121<\/jats:sup>\n                    ; NS). We concluded that 8-iso-PGF2\u03b1 stimulates cAMP and increases Cl transport in cTALs via a PKA-dependent mechanism.\n                  <\/jats:p>","DOI":"10.1152\/ajprenal.00225.2010","type":"journal-article","created":{"date-parts":[[2010,9,22]],"date-time":"2010-09-22T21:05:14Z","timestamp":1285189514000},"page":"F1396-F1400","source":"Crossref","is-referenced-by-count":8,"title":["8-iso-prostaglandin-F2\u03b1 stimulates chloride transport in thick ascending limbs: role of cAMP and protein kinase A"],"prefix":"10.1152","volume":"299","author":[{"given":"Pablo D.","family":"Cabral","sequence":"first","affiliation":[{"name":"Hypertension and Vascular Research Division, Department of Internal Medicine, Henry Ford Hospital, Detroit, Michigan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Renal Physiology, J. Robert Cade Foundation CONICET, Cordoba;"},{"name":"Department of Renal Physiology and Hypertension, Mons. Carlos V. Cruvellier Foundation, San Juan, Argentina; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra T.","family":"Baigorria","sequence":"additional","affiliation":[{"name":"Department of Renal Physiology, J. Robert Cade Foundation CONICET, Cordoba;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A.","family":"Juncos","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics and Division of Nephrology, Department of Medicine, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis I.","family":"Juncos","sequence":"additional","affiliation":[{"name":"Department of Renal Physiology, J. Robert Cade Foundation CONICET, Cordoba;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00e9stor H.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Renal Physiology, J. Robert Cade Foundation CONICET, Cordoba;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.2.C455"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90446.2008"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1404"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2005.7.236"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/348637a0"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1982.198"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.037135"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1977.232.4.F383"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1999.277.5.R1476"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00239.x"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.34.3.508"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1985.65.3.760"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.34.4.983"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00543.2009"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00447.2005"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.03.014"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1993.tb55605.x"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.37.2.541"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00346.2001"},{"key":"B20","doi-asserted-by":"crossref","first-page":"4317","DOI":"10.1016\/S0021-9258(17)41781-9","volume":"269","author":"Morrow JD","year":"1994","journal-title":"J Biol Chem"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.38.3.655"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00248.2005"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.3.F359"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118745"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.35.1.476"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.34.20617"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2760(96)00162-2"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.33.1.424"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1990.259.3.F408"},{"key":"B30","author":"Silva GB","year":"2006","journal-title":"J Am Soc Nephrol"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.108.120212"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00472.2009"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000236646.83354.51"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00021.2003"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00269.2004"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115790"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000136395.06810.cf"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00225.2010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T08:02:37Z","timestamp":1636531357000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00225.2010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1152\/ajprenal.00225.2010"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00225.2010","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.7084956.7308055","asserted-by":"object"}]},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12]]}}}